Analyses of Mouse Zbtb20 Expression Left and Middle Panels - Immunohistochemical analysis of Zbtb20 expression in mouse spleen and small intestine. Acetone-fixed, frozen spleen and small intestine sections were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 559073) or Purified Rat Anti-Mouse Zbtb20 antibody (Cat. No. 565453). A three-step staining procedure that employs a Biotin Goat Anti-Rat Immunoglobulin (Cat. No. 559286), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The 4A3 antibody primarily stained some lymphocytes in the splenic white and red pulp (Left Panel), and some cells in the lamina propria and myenteric plexus of the small intestine (Middle Panel). Original magnification: 20x. Right Panel - Flow cytometric analysis of Zbtb20 expression by mouse peritoneal cells. BALB/c mouse peritoneal cells were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with either Purified Rat IgG2a, κ Isotype Control (dashed line histogram) or Purified Rat Anti-Mouse Zbtb20 antibody (solid line histogram). The cells were washed then secondarily stained with PE Goat Anti-Rat Ig (Cat. No. 550767). The fluorescence histogram showing Zbtb20 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact peritoneal cells.
Analyses of Mouse Zbtb20 Expression Left and Middle Panels - Immunohistochemical analysis of Zbtb20 expression in mouse spleen and small intestine. Acetone-fixed, frozen spleen and small intestine sections were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 559073) or Purified Rat Anti-Mouse Zbtb20 antibody (Cat. No. 565453). A three-step staining procedure that employs a Biotin Goat Anti-Rat Immunoglobulin (Cat. No. 559286), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The 4A3 antibody primarily stained some lymphocytes in the splenic white and red pulp (Left Panel), and some cells in the lamina propria and myenteric plexus of the small intestine (Middle Panel). Original magnification: 20x. Right Panel - Flow cytometric analysis of Zbtb20 expression by mouse peritoneal cells. BALB/c mouse peritoneal cells were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with either Purified Rat IgG2a, κ Isotype Control (dashed line histogram) or Purified Rat Anti-Mouse Zbtb20 antibody (solid line histogram). The cells were washed then secondarily stained with PE Goat Anti-Rat Ig (Cat. No. 550767). The fluorescence histogram showing Zbtb20 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact peritoneal cells.
Product Details
BD Pharmingen™
DPZF; HOF; ODA-8S; Oda8; Zfp288
Mouse (QC Testing)
Rat IgG2a, κ
Mouse Zbtb20 Peptide
Intracellular staining (flow cytometry) (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
AB_2739244
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Transcription Factor Buffer Set RUO
Size
100 Tests
Cat No.
562574
Purified Rat IgG2a κ Isotype Control RUO
Size
0.25 mg
Cat No.
559073
Transcription Factor Buffer Set RUO
Size
25 Tests
Cat No.
562725
PE Goat Anti-Rat Ig RUO
Size
0.2 mg
Cat No.
550767
.png?imwidth=320)
565453 Rev. 1
Antibody Details
4A3
The 4A3 monoclonal antibody specifically binds to Zinc finger and BTB domain containing 20 (Zbtb20). This transcription factor is also known as Zinc finger protein 288 (Zfp288), BTB/POZ domain zinc finger factor HOF, BTB/POZ zinc finger protein DPZF, or POZ/zinc finger transcription factor ODA-8. Zbtb20 is a transcription factor that localizes to the nucleus and contains one BTB/POZ domain and 5 C2H2-type zinc fingers. Mouse ZBTB20 is expressed in thymus, spleen, lymph node, peritoneal cells and fetal liver. It is highly expressed in B1 and germinal center B cells, and long-lived antibody-secreting plasma cells. In cell lines, Zbtb20 is expressed at low levels in the immature WEHI231 and the mature 129B cell lines but at high levels in A20 cells, a germinal center B cell lymphoma. This transcription factor reportedly plays key roles in hematopoiesis, immunity, and oncogenesis.
565453 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
565453 Rev.1
Citations & References
Development References (2)
-
Chevrier S, Emslie D, Shi W, et al. The BTB-ZF transcription factor Zbtb20 is driven by Irf4 to promote plasma cell differentiation and longevity. J Exp Med. 2014; 211(5):827-840. (Immunogen: Flow cytometry, Western blot). View Reference
-
Xie Z, Ma X, Ji W, et al. Zbtb20 is essential for the specification of CA1 field identity in the developing hippocampus. Proc Natl Acad Sci U S A. 2010; 107(14):6510-6515. (Biology). View Reference
565453 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
