Three-color evaluation of splenic mononuclear phagocytes. Single-cell suspensions of Lewis splenocytes were stained with either purified 1C7 antibody (bottom panels) or isotype control (top panels), followed by biotinylated goat anti- mouse Ig, multiple adsorption (all panels, Cat. no.553999). In a third step (all panels), Streptavidin-PE-Cy5 (Cat. no. 554062) was added simultaneously with FITC-conjugated anti-rat mAb CD4 OX-35 (Cat. no. 554837) and PE-conjugated anti-rat RT1B mAb OX-6 (Cat. no. 554929). The percentages of cells with the phenotype of mononuclear phagocytes (CD4+ 1C7+ and RT1B+ 1C7+) are indicated in the boxed regions. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Three-color evaluation of splenic mononuclear phagocytes. Single-cell suspensions of Lewis splenocytes were stained with either purified 1C7 antibody (bottom panels) or isotype control (top panels), followed by biotinylated goat anti- mouse Ig, multiple adsorption (all panels, Cat. no.553999). In a third step (all panels), Streptavidin-PE-Cy5 (Cat. no. 554062) was added simultaneously with FITC-conjugated anti-rat mAb CD4 OX-35 (Cat. no. 554837) and PE-conjugated anti-rat RT1B mAb OX-6 (Cat. no. 554929). The percentages of cells with the phenotype of mononuclear phagocytes (CD4+ 1C7+ and RT1B+ 1C7+) are indicated in the boxed regions. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Product Details
BD Pharmingen™
Rat (QC Testing)
Mouse IgG1, κ
Not reported
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunohistochemistry-frozen (Reported)
0.5 mg/ml
AB_395612
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
Purified Mouse IgG1, κ Isotype Control RUO
Size
0.5 mg
Cat No.
557273
Biotin Goat Anti-Mouse Ig (Multiple Adsorption) RUO
Size
0.5 mg
Cat No.
553999
PE-Cy™5 Streptavidin RUO
Size
0.1 mg
Cat No.
554062
FITC Mouse Anti-Rat CD4 RUO
Size
0.5 mg
Cat No.
554837
PE Mouse Anti-Rat RT1B RUO
Size
0.2 mg
Cat No.
554929
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554954 Rev. 13
Antibody Details
1C7
The 1C7 monoclonal antibody reacts with an antigen found on almost all cells of the mononuclear phagocyte system. Its cellular distribution is reported to be identical to that of CD68 (ED1 Antigen), which has been detected on tissue macrophages, dendritic cells, osteoclasts, resident peritoneal macrophages, alveolar macrophages, and peripheral blood monocytes, but not on granulocytes or lymphocytes. The distribution of ED1+ myeloid cells is similar to that of RT1B (rat I-A equivalent), and all ED1+ cells have acid phosphatase activity.
554954 Rev. 13
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554954 Rev.13
Citations & References
Development References (3)
-
Dijkstra CD, Döpp EA, Joling P, Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985; 54(3):589-599. (Biology). View Reference
-
de Jong, M. W. A. The 1C7 antibody reacts with an antigen found on almost all cells of the mononuclear phagocyte system. .
-
van Goor H, Harms G, Gerrits PO, Kroese FG, Poppema S, Grond J. Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue. J Histochem Cytochem. 1988; 36(1):115-120. (Biology). View Reference
554954 Rev. 13
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
