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Purified Mouse Anti-Rat CD3
Purified Mouse Anti-Rat CD3

Immunohistochemical staining of T lymphocytes. The frozen section of normal rat spleen was reacted with Purified Mouse Anti-Rat CD3 (Cat. No. 550295). T lymphocytes in the periarteriolar sheath, are identified by the brown labeling of their cell surface membrane.

Immunohistochemical staining of T lymphocytes. The frozen section of normal rat spleen was reacted with Purified Mouse Anti-Rat CD3 (Cat. No. 550295). T lymphocytes in the periarteriolar sheath, are identified by the brown labeling of their cell surface membrane.

Product Details
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BD Pharmingen™
CD3 Complex; T3
Rat (QC Testing)
Mouse BALB/c IgG3, κ
PHA-stimulated rat lymph node and spleen cells
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Cytotoxicity, Immunoprecipitation, Inhibition, Stimulation (Reported)
31.25 µg/ml
Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: Purified Mouse Anti-Rat CD3 (Cat. No. 550295) is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and paraffin sections. Tissue tested was rat spleen. The antibody stains T lymphocytes. The isotype control recommended for use with this antibody is Purified Mouse IgG3, κ Isotype Control (Cat. No. 550341). For optimal indirect immunohistochemical staining, the G4.18 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Purified Mouse IgG3, κ Isotype Control (Cat. No. 553401) as the secondary antibody and Streptavidin HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure can be found at

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  6. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  7. Please refer to for technical protocols.
550295 Rev. 5
Antibody Details
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The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.

550295 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
550295 Rev.5
Citations & References
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Development References (3)

  1. Naper C, Vaage JT, Lambracht D, et al. Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control. Eur J Immunol. 1995; 25(5):1249-1256. (Clone-specific: Cytotoxicity). View Reference
  2. Nelson DJ, McMenamin C, McWilliam AS, Brenan M, Holt PG. Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med. 1994; 179(1):203-212. (Clone-specific: Immunohistochemistry). View Reference
  3. Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Immunogen: Cytotoxicity, Flow cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition, Stimulation). View Reference
550295 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.