Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
The CRF D-2712 monoclonal antibody specifically binds to mouse and rat CD36, an 88-kDa glycoprotein member of the class B scavenger receptor family. CD36 is a multifunctional receptor that has a variety of ligands and is expressed on many cell types. Consequently, it is known by several names, including macrophage scavenger receptor, platelet glycoprotein IV, and fatty acid translocase. Cells that express CD36 include platelets, megakaryocytes, monocytes/macrophages, dendritic cells, erythroid precursors, microvascular endothelia, skeletal, cardiac, and smooth muscle cells, adipocytes, and epithelia of retina, breast, and intestine. This multi-ligand receptor functions in the clearance of apoptotic cells and abnormal erythrocytes (in malaria and sickle-cell anemia), the transport of long-chain fatty acids and oxidized low-density lipoproteins, the regulation of angiogenesis by mediating the effects of thrombospondin-1, and the mediation of coronary vasoconstriction. Crosslinking of cell-surface CD36 with plate-bound CRF D-2712 antibody promotes internalization of photoreceptor outer segment fragments by cultured retinal pigment epithelium.
Development References (3)
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Bodart V, Febbraio M, Demers A, et al. CD36 mediates the cardiovascular action of growth hormone-releasing peptides in the heart. Circ Res. 2002; 90(8):844-849. (Biology). View Reference
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Febbraio M, Hajjar DP, Silverstein RL. CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J Clin Invest. 2001; 108(6):785-791. (Biology). View Reference
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Finnemann SC, Silverstein RL. Differential roles of CD36 and alphavbeta5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium. J Exp Med. 2001; 194(9):1289-1298. (Immunogen: Immunofluorescence, Immunoprecipitation). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
