Preparation And Storage
Recommended Assay Procedures
For optimal staining of peripheral lymphoid tissue, we recommend the use of Mouse BD Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), an isotype-specific second-step antibody, and amplification of the staining signal by use of a biotinylated second-step antibody (such as biotinylated anti-mouse IgG2b mAb R12-3, Cat. No. 553393) followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. No. 554061) or Streptavidin-APC (Cat. No. 554067).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
.png?imwidth=320)
.png?imwidth=320)
The BP-3 antibody reacts with CD157, also known as the BP-3 alloantigen or Bone marrow STromal cell antigen-1 (BST-1), found in a variety of common laboratory strains of mice (AKR, BALB/c, CBA/N, C3H/HeJ, C57BL/6, DBA/2, NZB, C.B-17, SJL and Swiss, but not A/J). CD157 is a differentially glycosylated GPI-linked cell-surface glycoprotein with a core protein of 32 kDa and differing patterns of glycosylation in different types of cells. It is a member, along with CD38, of a family of genetically-related ectoenzymes. The BP-3 alloantigen is expressed on the B lineage from the earliest detectable progenitors to circulating immature B lymphocytes, on immature thymocytes up to the expression of the T-cell receptor, on circulating neutrophils, peritoneal macrophages, on subpopulations of stromal cells in peripheral lymphoid organs (but not in bone marrow or thymus), on intestinal epithelial cells, and in the lumen of kidney-collecting tubules. The distribution of CD157 antigen on leukocytes and stromal cells of lymphoid organs suggests that it may be involved in leukocyte-stroma interactions which support the development, migration, and/or responses of leukocyte populations.
Development References (6)
-
Dong C, Wang J, Neame P, Cooper MD. The murine BP-3 gene encodes a relative of the CD38/NAD glycohydrolase family. Int Immunol. 1994; 6(9):1353-1360. (Clone-specific: Immunoaffinity chromatography). View Reference
-
Dong C, Willerford D, Alt FW, Cooper MD. Genomic organization and chromosomal localization of the mouse Bp3 gene, a member of the CD38/ADP-ribosyl cyclase family. Immunogenetics. 1996; 45(1):35-43. (Biology). View Reference
-
Itoh M, Ishihara K, Hiroi T, et al. Deletion of bone marrow stromal cell antigen-1 (CD157) gene impaired systemic thymus independent-2 antigen-induced IgG3 and mucosal TD antigen-elicited IgA responses. J Immunol. 1998; 161(8):3974-3983. (Biology). View Reference
-
McNagny KM, Bucy RP, Cooper MD. Reticular cells in peripheral lymphoid tissues express the phosphatidylinositol-linked BP-3 antigen. Eur J Immunol. 1991; 21(2):509-515. (Clone-specific: Immunoprecipitation). View Reference
-
McNagny KM, Cazenave PA, Cooper MD. BP-3 alloantigen. A cell surface glycoprotein that marks early B lineage cells and mature myeloid lineage cells in mice. J Immunol. 1988; 141(8):2551-2556. (Immunogen: Immunofluorescence, Immunoprecipitation). View Reference
-
Ngo VN, Korner H, Gunn MD, et al. Lymphotoxin alpha/beta and tumor necrosis factor are required for stromal cell expression of homing chemokines in B and T cell areas of the spleen. J Exp Med. 1999; 189(2):403-412. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
