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Purified Mouse Anti-Human Fetal Hemoglobin
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

We recommend to use 0.05% cold glutaraldehyde at room temperature for 10 minutes to fix the cells, then use 0.1% Triton X-100 at room temperature for 10 minutes to permeabilize the cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551796 Rev. 5
Antibody Details
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Reacts with fetal hemoglobin (HbF), a form of hemoglobin present during fetal development. HbF resembles adult hemoglobin (HbA) in possessing two alpha chains but differs in possessing two gamma instead of two beta chains. In normal adults, synthesis of HbF does persist at very low levels (<1% of total Hb) and is restricted to a small population of erythrocytes called F cells. Hemoglobin F-expressing erythrocytes are normally seen during pregnancy. An increase in the expression of fetal hemoglobin in adult peripheral red blood cells is a common feature in the genetic disorders of hemoglobin, sickle-cell disease (SCD) and beta thalassemia.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

551796 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
551796 Rev.5
Citations & References
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Development References (3)

  1. Campbell TA, Ware RE, Mason M. Detection of hemoglobin variants in erythrocytes by flow cytometry. Cytometry. 1999; 35(3):242-248. (Biology). View Reference
  2. Horiuchi K, Osterhout ML, Kamma H, Bekoe NA, Hirokawa KJ. Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry. Cytometry. 1995; 20(3):261-267. (Biology). View Reference
  3. Thorpe SJ, Thein SL, Sampietro M, Craig JE, Mahon B, Huehns ER. Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis. Br J Haematol. 1994; 87(1):125-132. (Biology). View Reference
551796 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.