Flow cytometric analysis of mouse GITR Ligand expression on non-transfected and GITR Ligand-transfected cells. Non-transfected (Left Panel) and GITR Ligand-transfected (Right Panel) P815 cells were stained with either PE Rat IgG2b, κ Isotype Control (Cat. No. 553989) or PE Rat Anti-Mouse GITR Ligand antibody (Cat. No. 563541). Fluorescence histograms showing the expression of GITR Ligand (solid line histograms) or Ig Isotype control staining (dashed line histograms) on non-transfected or GITR Ligand-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analyses were performed using a BD™ LSR II Flow Cytometer System.
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Flow cytometric analysis of mouse GITR Ligand expression on non-transfected and GITR Ligand-transfected cells. Non-transfected (Left Panel) and GITR Ligand-transfected (Right Panel) P815 cells were stained with either PE Rat IgG2b, κ Isotype Control (Cat. No. 553989) or PE Rat Anti-Mouse GITR Ligand antibody (Cat. No. 563541). Fluorescence histograms showing the expression of GITR Ligand (solid line histograms) or Ig Isotype control staining (dashed line histograms) on non-transfected or GITR Ligand-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analyses were performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
Tnfsf18; Gitrl Glucocorticoid-induced-tumor necrosis factor receptor ligand
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Mouse GITRL Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
240873
AB_2738269
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Rat IgG2b, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553989
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563541 Rev. 1
Antibody Details
MIH44
The MIH44 monoclonal antibody specifically binds to Glucocorticoid-induced TNF-related Ligand (GITR Ligand). The GITR Ligand protein is encoded by the Tnfsf18 (Tumor necrosis factor ligand superfamily member 18) gene. The GITR Ligand is a type II transmembrane protein that is expressed on endothelial cells and antigen presenting cells, including B cells, macrophage and dendritic cells. The GITR Ligand reportedly costimulates T cell activation and reverses CD4+CD25+ Treg cell-mediated suppression by interaction with its cognate receptor, GITR that is expressed by these cells.
563541 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563541 Rev.1
Citations & References
Development References (2)
-
Igarashi H, Cao Y, Iwai H, et al. GITR ligand-costimulation activates effector and regulatory functions of CD4+ T cells. Biochem Biophys Res Commun. 2008; 369(4):1134-1138. (Immunogen: Blocking, Flow cytometry, Inhibition). View Reference
-
Piao J, Kamimura Y, Iwai H, et al. Enhancement of T-cell-mediated anti-tumour immunity via the ectopically expressed glucocorticoid-induced tumour necrosis factor receptor-related receptor ligand (GITRL) on tumours. Immunology. 2009; 127(4):489-499. (Clone-specific: Flow cytometry). View Reference
563541 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
