Product Details
BD Pharmingen™
Il2rg; IL-2Rγ; Commin gamma chain; Common γ; gamma c receptor; γc
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2b, κ
Mouse IL-2Rγ Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_395415
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
PE- conjugated clone TUGm2 can be used for immunofluorescent staining with flow cytometric analysis. An appropriate isotype control is Cat. No. 553989. The no azide/low endotoxin format of the TUGm2 antibody, useful for blocking or neutralization studies, is Cat. No. 554469.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554471 Rev. 2
Antibody Details
TUGm2
The TUGm2 monoclonal antibody specifically binds to CD132, which is also known as, the common γ subunit (γc). The γc chain subunit is shared by the IL-2, IL-4, IL-7, IL-9, and IL-15 receptor complexes. The γc receptor is expressed constitutively at low levels by most lymphocytes, myeloid cells, embryonic thymocytes, and lymphoid cell lines. The γc receptor is a 75-80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated. TUGm2 antibody can significantly block the binding of and proliferative response to IL-4 by CTLL-2 cells. It can also inhibit binding to high-affinity IL-2 receptors expressed by CTLL-2 cells. By itself, the TUGm2 antibody reportedly fails to significantly inhibit CTLL-2 proliferative responses to IL-2. However, when added along with TM-β1 (Cat. No. 553359), specific for the mouse IL-2Rβ chain, TUGm2 was shown to synergistically reduce IL-2-driven CTLL-2 proliferative responses. TUGm2 can reduce the IL-7-induced proliferation of Con A-activated mouse splenocytes and the IL-7 responsive pre-B cell line, IxN/2b.
554471 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
554471 Rev.2
Citations & References
View product citations for antibody "554471" on CiteAb
Development References (2)
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Kondo M, Takeshita T, Higuchi M, et al. Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes. Science. 1994; 263(5152):1453-1454. (Clone-specific). View Reference
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Kondo M, Takeshita T, Ishii N, et al. Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. Science. 1993; 262(5141):1874-1877. (Clone-specific: Blocking, Neutralization). View Reference
554471 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.