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PE Mouse anti-Human TRA-1-85 Antigen
PE Mouse anti-Human TRA-1-85 Antigen
Flow cytometric analysis of TRA-1-85 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (solid line histogram) or with PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dashed line histogram). Red blood cells were then lysed using BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Flow cytometric analysis of TRA-1-85 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (solid line histogram) or with PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dashed line histogram). Red blood cells were then lysed using BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Product Details
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BD Pharmingen™
OKa, CD147, EMMPRIN, Basigin
Human (QC Testing)
Mouse IgG1, κ
Human Embryonal Carcinoma Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2737957
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563021 Rev. 1
Antibody Details
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TRA-1-85

The TRA-1-85 monoclonal antibody (mAb) specifically binds to the high frequency blood group antigen OKa. The OKa blood group antigen is a 35-69 kDa glycoprotein also known as CD147, EMMPRIN, M6, or Basigin. The OKa group is widely expressed on most human tissues including, but not limited to kidney, peripheral blood leukocytes, liver, pancreas, colon, skin, and brain. TRA-1-85 mAb is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies. During development, the purified TRA-1-85 mAb was found to detect OKa blood group antigen by western blot analysis of cellular lysates, by immunofluorescent staining of fixed cells, and by flow cytometric analysis of live cells.

563021 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563021 Rev.1
Citations & References
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Development References (5)

  1. Choi KD, Yu J, Smuga-Otto K, et al. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells. Stem Cells. 2009; 27(3):559-567. (Clone-specific: Flow cytometry). View Reference
  2. Draper JS, Pigott C, Thomson JA, Andrews PW. Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat. 2002; 200:249-258. (Clone-specific: Flow cytometry). View Reference
  3. Olivier EN, Rybicki AC, Bouhassira EE. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells. 2006; 24(8):1914-1922. (Biology). View Reference
  4. Spring FA, Holmes CH, Simpson KL, et.al. The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, basigin and neurothelin, an immunoglobulin superfamily molecule that is widely expressed in human cells and tissues. Eur J Immunol. 1997; 27(4):891-897. (Clone-specific: Flow cytometry, Immunohistochemistry, Western blot). View Reference
  5. Williams BP, Daniels GL, Pym B, et al. Biochemical and genetic analysis of the OKa blood group antigen. Immunogenetics. 1988; 27:322-329. (Clone-specific: Flow cytometry). View Reference
View All (5) View Less
563021 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.