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Two-parameter flow cytometric analysis of ILT1 (CD85h) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human ILT1 (CD85h) antibody (Cat. No. 566577; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of ILT1 (CD85h) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human ILT1 (CD85h)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The monoclonal antibody 24 specifically recognizes Immunoglobulin-like transcript 1 (ILT1) which is also known as Leukocyte immunoglobulin-like receptor 7 (LIR-7). ILT1 (CD85h) is encoded by LILRA2 (Leukocyte immunoglobulin like receptor A2) and belongs to the Leukocyte immunoglobulin like receptors (LILR) gene family. This immunoreceptor is a ~69 kDa type I transmembrane glycoprotein that contains 4 Ig-like C2-type immunoglobulin-like extracellular domains and a short cytoplasmic domain. It is expressed on monocytes, macrophages, myeloid dendritic cells, and at lower levels on some granulocytes and a small subset of natural killer (NK) cells. ILT1 (CD85h) serves as an activating immunoreceptor through its short cytoplasmic domain that associates with the signal-transducing Fc receptor γ chain. This receptor complex mediates suppression of innate immune responses and inhibition of dendritic cell differentiation and antigen presentation. ILT1 (CD85h) may act as a receptor for immunoglobulins cleaved by microbial proteases.
Development References (5)
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Hirayasu K, Saito F, Suenaga T, et al. Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2. Nat Microbiol. 2016; 1(6):16054. (Biology). View Reference
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Lee DJ, Sieling PA, Ochoa MT, et al. LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007; 179(12):8128-8136. (Immunogen: Functional assay, Immunofluorescence). View Reference
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Lichterfeld M, Yu XG. The emerging role of leukocyte immunoglobulin-like receptors (LILRs) in HIV-1 infection. J Leukoc Biol. 2012; 91(1):27-33. (Biology). View Reference
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Nakajima H, Samaridis J, Angman L, Colonna M. Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor gamma-chain.. J Immunol. 1999; 162(1):5-8. (Biology). View Reference
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Tedla N, Bandeira-Melo C, Tassinari P, et al. Activation of human eosinophils through leukocyte immunoglobulin-like receptor 7. Proc Natl Acad Sci U S A. 2003; 100(3):1174-1179. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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