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PE Mouse Anti-Human CX3CL1 (Fractalkine)
PE Mouse Anti-Human CX3CL1 (Fractalkine)
Flow cytometric analysis of CX3CL1 expression on wild type (Left Panel) or Crispr/Cas9 knockout (Right Panel) HepG2 cells. Wild type or knockout human HepG2 cells were detached with Accutase (Cat. No. 561527) and stained with either Mouse Anti-Human CX3CL1 antibody (Cat. No. 566905; solid line histogram) or PE mIgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of live cells. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Crispr/Cas9 knockout HepG2 was generated by Synthego Corp. Knockout HepG2 cells were bulk sorted and sorted cells were expanded by culturing before staining. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CX3CL1 expression on wild type (Left Panel) or Crispr/Cas9 knockout (Right Panel) HepG2 cells. Wild type or knockout human HepG2 cells were detached with Accutase (Cat. No. 561527) and stained with either Mouse Anti-Human CX3CL1 antibody (Cat. No. 566905; solid line histogram) or PE mIgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of live cells. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Crispr/Cas9 knockout HepG2 was generated by Synthego Corp. Knockout HepG2 cells were bulk sorted and sorted cells were expanded by culturing before staining. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
ABCD-3; C-X3-C motif chemokine 1; CXC3; CXC3C; NTN; NTT; SCYD1; neurotactin
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CX3CL1 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2869945
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566905 Rev. 1
Antibody Details
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V13-864

The V13-864 monoclonal antibody specifically recognizes CX3C motif chemokine Ligand 1 (CX3CL1), which is also known as fractalkine. CX3CL1 (Fractalkine) is the only known member of the CX3C chemokine subfamily and is expressed as both membrane-bound and secreted forms. Unlike other chemokines, CX3CL1is expressed on the surface of endothelial cells and not significantly expressed on peripheral blood leukocytes.  CX3CL1's receptor is known as CX3CR1 and is expressed on cytotoxic T cells, NK cells, dendritic cells, and monocytes. Overexpression of CX3CL1 (Fractalkine) in some malignant tumors may contribute to the recruitment of effectors for innate and adaptive immunity.

566905 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566905 Rev.1
Citations & References
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Development References (5)

  1. Bazan JF, Bacon KB, Hardiman G, et al. A new class of membrane-bound chemokine with a CX3C motif.. Nature. 1997; 385(6617):640-4. (Biology). View Reference
  2. Colobran R, Pujol-Borrell R, Armengol MP, Juan M. The chemokine network. I. How the genomic organization of chemokines contains clues for deciphering their functional complexity.. Clin Exp Immunol. 2007; 148(2):208-17. (Biology). View Reference
  3. Park MH, Lee JS, Yoon JH. High expression of CX3CL1 by tumor cells correlates with a good prognosis and increased tumor-infiltrating CD8+ T cells, natural killer cells, and dendritic cells in breast carcinoma. J Surg Oncol. 2012; 106(4):386-92. (Biology). View Reference
  4. Zlotnik A, Yoshie O, Nomiyama H. The chemokine and chemokine receptor superfamilies and their molecular evolution.. Genome Biol. 2006; 7(12):243. (Biology). View Reference
  5. Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity.. Immunity. 2000; 12(2):121-7. (Biology). View Reference
View All (5) View Less
566905 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.