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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The DF-T1 monoclonal antibody specifically recognizes CD43 which is also known as Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, Galactoglycoprotein (GALGP), or Ly-48. CD43 is an ~95-135 kDa heavily O-sialylated type I transmembrane glycoprotein that is encoded by SPN which belongs to the cell surface mucin family. CD43 is highly expressed on T lymphocytes, thymocytes, monocytes, granulocytes, bone marrow stem cells, pre-B cells, activated B cells and plasma cells but not on resting peripheral blood B cells, red blood cells, and nonhematopoietic cells. CD43 binds to CD54 (ICAM-1), CD62E (E-selectin), CD169 (Siglec-1), CD328 (Siglec-7), and MHC Class I molecules. It mediates intercellular interactions that regulate leukocyte functions including cellular adhesion and anti-adhesion, lectin binding, co-stimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. Enzymatic shedding of CD43 from leukocyte surfaces following their activation by various stimuli may play a negative regulatory role in adaptive immune responses.
Development References (9)
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Larbi A, Gombert JM, Auvray C, et al. The HOXB4 homeoprotein promotes the ex vivo enrichment of functional human embryonic stem cell-derived NK cells.. PLoS One. 2012; 7(6):e39514. (Clone-specific: Flow cytometry). View Reference
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McGowan P, Nelles N, Wimmer J, et al. Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma: the role of commonly used flow cytometry cell markers and the application of a multiparameter scoring system.. Am J Clin Pathol. 2012; 137(4):665-70. (Clone-specific: Flow cytometry). View Reference
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Morland BJ, Barley J, Boehm D, et al. Effectiveness of HB2 (anti-CD7)--saporin immunotoxin in an in vivo model of human T-cell leukaemia developed in severe combined immunodeficient mice.. Br J Cancer. 1994; 69(2):279-85. (Clone-specific: Immunofluorescence). View Reference
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Sandoval-Hernández MA, Fierro NA, Veytia-Bucheli JI, et al. Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR.. Int J Mol Sci. 2024; 25(6):3135. (Clone-specific). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Stross WP, Warnke RA, Flavell DJ, et al. Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen.. J Clin Pathol. 1989; 42(9):953-61. (Immunogen: Flow cytometry, Western blot). View Reference
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Thiemann S, Man JH, Chang MH, Lee B, Baum LG. Galectin-1 regulates tissue exit of specific dendritic cell populations.. J Biol Chem. 2015; 290(37):22662-77. (Clone-specific: Flow cytometry, Immunofluorescence, Western blot). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.