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PE Mouse anti-Human CD39
PE Mouse anti-Human CD39

Flow cytometric analysis of PE Mouse anti-Human CD39 on peripheral blood. Human peripheral blood was isolated by Ficoll gradient and stained simultaneously with PerCP CD4 (clone SK3, Cat. No. 340671), Alexa Fluor 647 CD127 (clone hIL7R-M21, Cat. No. 558598) and PE CD39 (clone TU66, Cat. No. 555464).  The cells were then fixed and permeabilized using the Foxp3 buffer kit (Cat. No. 560098)  and then stained with Alexa Fluor 488 Foxp3 (clone 259D, Cat. No. 560047).The dot plot (data not shown) was derived from gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4 positive cells.  Regulatory T cells were then identified by the CD127 versus Foxp3 staining pattern (right panel) and analyzed for CD39 expression (left panel).  Flow cytometry was performed on a BD FACSCanto™ System.

Flow cytometric analysis of PE Mouse anti-Human CD39 on peripheral blood. Human peripheral blood was isolated by Ficoll gradient and stained simultaneously with PerCP CD4 (clone SK3, Cat. No. 340671), Alexa Fluor 647 CD127 (clone hIL7R-M21, Cat. No. 558598) and PE CD39 (clone TU66, Cat. No. 555464).  The cells were then fixed and permeabilized using the Foxp3 buffer kit (Cat. No. 560098)  and then stained with Alexa Fluor 488 Foxp3 (clone 259D, Cat. No. 560047).The dot plot (data not shown) was derived from gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4 positive cells.  Regulatory T cells were then identified by the CD127 versus Foxp3 staining pattern (right panel) and analyzed for CD39 expression (left panel).  Flow cytometry was performed on a BD FACSCanto™ System.

Product Details
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BD Pharmingen™
ENTPD1; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
Human (QC Testing)
Mouse IgG2b, κ
Flow cytometry (Routinely Tested)
20 µl
IV A54
953
AB_395856
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
555464 Rev. 4
Antibody Details
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TU66

The TU66 monoclonal antibody specifically recognizes human CD39 which is also known as Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), Ecto-ATP diphosphohydrolase 1 (Ecto-ATPDase 1), or Ecto-apyrase. CD39 is an integral membrane glycoprotein with two transmembrane domains, N- and C-terminal cytoplasmic tails, and an extracellular region that contains the NTPDase 1 active site. CD39 is encoded by ENTPD1 which belongs to the ectoenzyme family. CD39 is variably expressed on activated T cells and B cells, regulatory T cells (Treg), dendritic cells, Langerhans cells, NK cells, monocytes, macrophages, endothelial cells, and granulocytes. CD39 acts on extracellular nucleoside triphosphates and diphosphates including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.

555464 Rev. 4
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555464 Rev.4
Citations & References
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Development References (4)

  1. Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.. Blood. 2007. (Biology). View Reference
  2. Duensing S, Kirshner H, Atzpodien J. CD39 as a novel marker of in vivo immune activation. Blood. 1994; 83(12):3826-3827. (Biology). View Reference
  3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
View All (4) View Less
555464 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.