Skip to main content Skip to navigation
PE Mouse Anti-Human CD318 (CDCP1)
PE Mouse Anti-Human CD318 (CDCP1)
Flow cytometric analysis of CD318 expression on human colorectal adenocarcinoma cell line. HT-29 cells (ATCC HTB-38) were stained with PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; dashed line) or PE Mouse Anti-Human CD318 (CDCP1) (Cat. No. 566656; solid line) at 0.5 µg/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CD318 expression on human colorectal adenocarcinoma cell line. HT-29 cells (ATCC HTB-38) were stained with PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; dashed line) or PE Mouse Anti-Human CD318 (CDCP1) (Cat. No. 566656; solid line) at 0.5 µg/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
CUB domain-containing protein 1; SIMA135; TRASK; membrane glycoprotein gp140
Human (QC Testing)
Mouse BALB/c IgG2b, κ
Human CDCP1 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869813
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566656 Rev. 1
Antibody Details
Down Arrow Up Arrow
CUB1

The CUB1 monoclonal antibody specifically reacts with CUB domain-containing protein 1 (CDCP1 or CD318), which is expressed on primary or cultured pluripotent stem cells (eg ESC, iPSC, HSC, MSC, and NSC). It is not detected on normal mature hematopoietic cells of the peripheral blood, but it is found on leukemia and lymphoma cells. CDCP1 is a type I transmembrane protein with three extracellular CUB domains and five potential tyrosine phosphorylation sites in the cytoplasm. CUB (C1r/C1s, Uegf, Bmp) domains are ~110-amino-acid motifs almost exclusively found in developmentally regulated proteins that are involved in a variety of inter- or extracellular interactions. For example, CDCP1 is upregulated on some cancers and neoplastic cell lines and is involved in the control of tumor cell proliferation and metastasis.

566656 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566656 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Bühring HJ, Kuçi S, Conze T, et al. CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and nonhematopoietic origin. Stem Cells. 2004; 22(3):334-343. (Immunogen: Flow cytometry). View Reference
  2. Conze T, Lammers R, Kuci S, et al. CDCP1 is a novel marker for hematopoietic stem cells. Ann N Y Acad Sci. 2003; 996:222-226. (Clone-specific: Flow cytometry). View Reference
  3. Hooper JD, Zijlstra A, Aimes RT, et al. Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 2003; 22(12):1783-94. (Biology). View Reference
  4. Ikeda JI, Morii E, Kimura H, et al. Epigenetic regulation of the expression of the novel stem cell marker CDCP1 in cancer cells. J Pathol. 2006; 210(1):75-84. (Biology). View Reference
  5. Miyazawa Y, Uekita T, Hiraoka N, et al. CUB domain-containing protein 1, a prognostic factor for human pancreatic cancers, promotes cell migration and extracellular matrix degradation. Cancer Res. 2010; 70(12):5136-46. (Biology). View Reference
View All (5) View Less
566656 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.