The 2-69 monoclonal antibody specifically binds to 2B4 which is also known as CD244. CD244 is an approximately 63-70 kDa type I transmembrane glycoprotein. It is a SLAM submember (SLAMF4) and CD2 family member of the immunoglobulin superfamily. CD244 was originally identified in the mouse as a non-MHC-restricted cytotoxicity mediator present on NK cells and CD8+ T cells. In humans, CD244 is expressed on NK cells, γδ T cells, subsets of effector and memory CD8+ T cells, monocytes, eosinophils and basophils. This expression pattern suggests a broad role for CD244 in the regulation of leukocyte activation. CD244 binds CD48 with high affinity. CD244 may serve both stimulatory and inhibitory functions depending on a number of factors. These include the intensity and duration of CD244 interactions with its ligands as well as the presence of other signals generated through different receptor-ligand interactions. 2B4 was clustered as CD244 in the VIIth HLDA workshop.
This antibody is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg, 610/20-nm filter).