BD Horizon™ BV480 Goat Anti-Rabbit IgG is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rabbit IgG primary antibodies. It reacts with rabbit IgG and the light chains of other rabbit immunoglobulins. BV480 Anti-Rabbit IgG has minimal crossreactivity with human, mouse, or rat serum proteins. As a second step staining reagent, it is reactive with rabbit polyclonal and monoclonal antibodies.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.