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      BV421 Mouse Anti-Mouse Allergin-1
      BV421 Mouse Anti-Mouse Allergin-1
      Flow cytometric analysis of Allergin-1 expression on mouse CD117+ (mast cells) peritoneal exudate cells (far left histogram). BALB/c peritoneal exudate cells (PECs) were stained with APC Rat Anti-Mouse CD117 antibody (Cat. No. 553356/561074) and 1 μg/test of either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dotted line histogram) or BD Horizon BV421 Mouse Anti-Mouse Allergin-1 antibody (Cat. No. 566340; solid line histogram). The fluorescence histogram showing Allergin-1 expression (or Ig Isotype control staining) was derived from CD117-positive events with the forward and side light-scattered characteristics of viable PECs. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not specific. Multicolor flow cytometric analysis of Allergin-1 expression on mouse splenic leucocyte populations (middle left, middle right, far right). BALB/c mouse splenic leucocytes were stained with PE Rat Anti-Mouse CD11b (Cat. No. 553311/557397/561689) and APC Hamster Anti-Mouse CD11c (Cat. No. 550261/561119) antibodies, and 1.0 μg/test of either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dotted line histograms) or BV421 Mouse Anti-Mouse Allergin-1 antibody (Cat. No. 566340; solid line histograms). Histograms showing Allergin-1 expression (or Ig Isotype control staining) were derived from CD11b+CD11c+ (dendritic cells) or CD11b+CD11c- (monocytes/macrophages) gated events with the forward and side light-scattered characteristics of viable leucocyte populations as indicated. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Mouse Anti-Mouse Allergin-1
      Flow cytometric analysis of Allergin-1 expression on mouse CD117+ (mast cells) peritoneal exudate cells (far left histogram). BALB/c peritoneal exudate cells (PECs) were stained with APC Rat Anti-Mouse CD117 antibody (Cat. No. 553356/561074) and 1 μg/test of either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dotted line histogram) or BD Horizon BV421 Mouse Anti-Mouse Allergin-1 antibody (Cat. No. 566340; solid line histogram). The fluorescence histogram showing Allergin-1 expression (or Ig Isotype control staining) was derived from CD117-positive events with the forward and side light-scattered characteristics of viable PECs. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not specific. Multicolor flow cytometric analysis of Allergin-1 expression on mouse splenic leucocyte populations (middle left, middle right, far right). BALB/c mouse splenic leucocytes were stained with PE Rat Anti-Mouse CD11b (Cat. No. 553311/557397/561689) and APC Hamster Anti-Mouse CD11c (Cat. No. 550261/561119) antibodies, and 1.0 μg/test of either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dotted line histograms) or BV421 Mouse Anti-Mouse Allergin-1 antibody (Cat. No. 566340; solid line histograms). Histograms showing Allergin-1 expression (or Ig Isotype control staining) were derived from CD11b+CD11c+ (dendritic cells) or CD11b+CD11c- (monocytes/macrophages) gated events with the forward and side light-scattered characteristics of viable leucocyte populations as indicated. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      Allergy inhibitory receptor 1; Milr1; Mast cell antigen 32; Mca32; Gm885
      Mouse (QC Testing)
      Mouse IgG1, κ
      Mouse Allergin-1 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2739697
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566340 Rev. 1
      Antibody Details
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      TX83

      The TX83 monoclonal antibody specifically recognizes Allergin-1 which is also known as Allergy inhibitory receptor 1 or Mast cell antigen 32 (Mast cell Ag-32/ Mca32). Allergin-1 is a ~60 kDa type I transmembrane glycoprotein that is encoded by Milr1 (Mast cell immunoglobulin like receptor 1) and contains two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. Allergin-1 is expressed on mast cells as well as some dendritic cells, macrophages, and neutrophils. When ligated with FcεRI, Allergin-1 inhibits FcεRI-mediated degranulation of mast cells which suppresses immediate hypersensitvity reactions.

      The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

      566340 Rev. 1
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      566340 Rev.1
      Citations & References
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      Development References (2)

      1. Hitomi K, Tahara-Hanaoka S, Someya S, et al. An immunoglobulin-like receptor, Allergin-1, inhibits immunoglobulin E-mediated immediate hypersensitivity reactions.. Nat Immunol. 2010; 11(7):601-7. (Immunogen: Flow cytometry, Functional assay). View Reference
      2. Nagai K, Tahara-Hanaoka S, Morishima Y, et al. Expression and function of Allergin-1 on human primary mast cells.. PLoS ONE. 2013; 8(10):e76160. (Biology). View Reference
      566340 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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