BD Horizon™ BV421 Goat Anti-Rabbit IgG is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rabbit IgG primary antibodies. It reacts with rabbit IgG and the light chains of other rabbit immunoglobulins. BV421 Anti-Rabbit IgG has minimal crossreactivity with human, mouse, or rat serum proteins. As a second step staining reagent, it is reactive with rabbit polyclonal and monoclonal antibodies.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. BD Horizon BV421 has an Ex Max at 407 nm and Em Max at 421 nm. The use of a mounting reagent (eg, ProLong® Gold) is highly recommended to maximize the photostability of BV421.
For confocal microscopy systems, a 405 nm laser is the optimal excitation source with optimal emission collection centered at 421 nm. For epifluorescence microscopes with broad spectrum excitation sources, the recommended excitation and emission filters are 392/23 nm and 430/24 nm bandpass filters, respectively. For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user. For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging.