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Biotin Rat Anti-Mouse IgG3
Product Details
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BD Pharmingen™
Ighg3; Immunoglobulin heavy constant gamma 3
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Mouse Pooled Ig
ELISA (Routinely Tested), Flow cytometry (Tested During Development), Immunohistochemistry-frozen (Reported)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

For the sandwich mouse IgG3, ELISA, Biotin Rat Anti-Mouse IgG3 is optimal for detection with Purified Rat Anti-Mouse IgG3 (Cat. No. 553404) for capture and Purified Mouse IgG3, κ Isotype Control (Cat. No. 553486) for the standard. Biotin Rat Anti-Mouse IgG3 may also be used as a primary or secondary reagent in immunofluorescent staining. Biotin Rat Anti-Mouse IgG3 is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG3, we recommend FITC Rat Anti-Mouse IgG3 (Cat. No. 553403).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Please refer to for technical protocols.
553401 Rev. 15
Antibody Details
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The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.

553401 Rev. 15
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553401 Rev.15
Citations & References
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Development References (2)

  1. Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. (Biology). View Reference
  2. Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. (Biology). View Reference
553401 Rev. 15

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.