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Biotin Mouse Anti-Mouse Vβ 5.1, 5.2 T-Cell Receptor
Biotin Mouse Anti-Mouse Vβ 5.1, 5.2 T-Cell Receptor

Two-color flow cytometric analysis of the expression of Vβ 5.1, 5.2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with Biotin Mouse Anti-Mouse Vβ 5.1, 5.2 T-Cell Receptor (Cat. No. 553188), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

Two-color flow cytometric analysis of the expression of Vβ 5.1, 5.2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with Biotin Mouse Anti-Mouse Vβ 5.1, 5.2 T-Cell Receptor (Cat. No. 553188), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

Product Details
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BD Pharmingen™
TCR Vβ5.1/5.2; Vβ5 T-cell receptor; T cell receptor beta 5
Mouse (QC Testing)
Mouse SWR IgG1, κ
Mouse T-Cell Hybridoma 2HB51.8
Flow cytometry (Routinely Tested)
0.5 mg/ml
21577
AB_394696
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553188 Rev. 15
Antibody Details
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MR9-4

The MR9-4 monoclonal antibody specifically recognizes the Vβ 5.1 and Vβ 5.2 T-cell Receptors of strains having the b haplotype (e.g., C57BL) of the Tcrb gene complex. These gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) Tcrb haplotype. Vβ5.1 and 5.2 TCR-bearing T lymphocytes are clonally eliminated, either completely or partially, in mice expressing I-E and superantigens encoded by the Mtv-1 (Mls-4a, Mlsc), Mtv-3 (Mlsc), Mtv-8 (Mlsf), Mtv-9 (Etc-1, Mlsf), Mtv-11 (Mlsf), Mtv-13 (Mls-2a, Mlsc), Mtv-27, Mtv44 , and/or Mtv-MAI endogenous provirus (e.g., A, AKR, BALB/c, C3H/He, C58, CBA/Ca, CBA/J, DBA/2, NZB, NZW). Activation of Vβ5 TCR-expressing T cells by this determinant is dependent upon presentation by I-E. Plate-bound MR9-4 antibody activates Vβ5.1 or 5.2 TCR-bearing T cells.

553188 Rev. 15
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553188 Rev.15
Citations & References
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Development References (9)

  1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). View Reference
  2. Bill J, Kanagawa O, Linten J, Utsunomiya Y, Palmer E. Class I and class II MHC gene products differentially affect the fate of V beta 5 bearing thymocytes. J Mol Cell Immunol. 1990; 4(5):269-280. (Immunogen). View Reference
  3. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
  4. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology). View Reference
  5. Hugin AW, Vacchio MS, Morse HC 3rd. A virus-encoded "superantigen" in a retrovirus-induced immunodeficiency syndrome of mice. Science. 1991; 252(5004):424-427. (Biology). View Reference
  6. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
  7. Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991; 21(4):1007-1011. (Biology). View Reference
  8. Woodland D, Happ MP, Bill J, Palmer E. Requirement for cotolerogenic gene products in the clonal deletion of I-E reactive T cells. Science. 1990; 247(4945):964-967. (Biology). View Reference
  9. Woodland DL, Happ MP, Gollob KJ, Palmer E. An endogenous retrovirus mediating deletion of alpha beta T cells. Nature. 1991; 349(6309):529-530. (Biology). View Reference
View All (9) View Less
553188 Rev. 15

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.