Multicolor flow cytometric analysis of Leptin Receptor (CD295) on human mononuclear cells. Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were washed and stained with FITC Mouse Anti-Human CD14 (Cat No. 555397/557153/561712), BD Horizon™ V450 Mouse Anti-Human CD19 (Cat No. 644491) antibodies and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat No. 557903) or Alexa Fluor® 647 Mouse Anti-Human Leptin Receptor (CD295) antibody (Cat No. 564376). The flow cytometric dot plots show the correlated expression patterns of CD19 (Upper Panels) or CD14 (Lower Panels) versus Leptin Receptor (CD295) [or Ig Isotype control staining] for gated events with the forward and side light-scatter characteristics of viable mononuclear leucocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Leptin Receptor (CD295) on human mononuclear cells. Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were washed and stained with FITC Mouse Anti-Human CD14 (Cat No. 555397/557153/561712), BD Horizon™ V450 Mouse Anti-Human CD19 (Cat No. 644491) antibodies and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat No. 557903) or Alexa Fluor® 647 Mouse Anti-Human Leptin Receptor (CD295) antibody (Cat No. 564376). The flow cytometric dot plots show the correlated expression patterns of CD19 (Upper Panels) or CD14 (Lower Panels) versus Leptin Receptor (CD295) [or Ig Isotype control staining] for gated events with the forward and side light-scatter characteristics of viable mononuclear leucocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
LEPR; Leptin receptor; LEP-R; OB receptor; OBR; HuB219
Human (QC Testing)
Mouse IgG2b, κ
Human Leptin Receptor Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2738777
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO
Size
100 Tests
Cat No.
557903
FITC Mouse Anti-Human CD14 RUO
Size
100 Tests
Cat No.
555397
FITC Mouse Anti-Human CD14 RUO
Size
50 Tests
Cat No.
557153
564376 Rev. 1
Antibody Details
52263
The 52263 monoclonal antibody specifically binds to the Leptin Receptor (Leptin R/LEPR) which is also known as CD295. CD295 is a type I transmembrane glycoprotein that belongs to the type I Cytokine Receptor family within the Immunoglobulin gene superfamily. Multiple isoforms of CD295 have been detected including a long form with a large cytoplasmic domain capable of signal transduction. Several receptor isoforms with short cytoplasmic domains that lack the capacity to transduce signals have also been reported. CD295 is widely expressed by a variety of cells within different tissues. CD295 binds to leptin with high affinity and plays important roles in lipid metabolism, fertility, angiogenesis, bone formation and immunity.
564376 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564376 Rev.1
Citations & References
Development References (3)
-
Fantuzzi G, Faggioni R. Leptin in the regulation of immunity, inflammation, and hematopoiesis. J Leukoc Biol. 2000; 68(4):437-446. (Biology). View Reference
-
Tartaglia LA, Dembski M, Weng X, et al. Identification and expression cloning of a leptin receptor, OB-R. Cell. 1995; 83(7):1263-1271. (Biology). View Reference
-
Venken K, Seeuws S, Zabeau L, et al.. A bidirectional crosstalk between iNKT cells and adipocytes mediated by leptin modulates susceptibility for T cell mediated hepatitis. J Hepatol. 2014; 60(1):175-182. (Biology). View Reference
564376 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
