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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products






The 406288 monoclonal antibody specifically recognizes DNAX-activation Protein 12 (DAP12) which is also known as Killer-activating receptor-associated protein (KARAP) or PLOSL. DAP12 is encoded by TYROBP (TYRO protein tyrosine kinase binding protein). The 12 kDa DAP12 protein contains a short extracellular domain, a transmembrane domain, and a cytoplasmic domain that contains a single immunoreceptor tyrosine-based activation motif (ITAM). DAP12 exists as a disulfide-linked homodimeric protein that is expressed by a variety of hematopoietic cells, including NK cells, monocytes, macrophages, dendritic cells, neutrophils, eosinophils, basophils, mast cells, and some NKT cells and γδ T cells. DAP12 functions as a signal transducing adaptor protein that associates with a variety of receptors involved in the detection of pathogens or host damage, including CD158 members of the Killer cell immunoglobulin like receptors (KIR) gene family, CD94-NKG2C and CD94-NKG2E heterodimeric receptors, Triggering receptor expressed on monocytes 1 (TREM-1) and TREM-2, and the NKp44 (CD336) receptor. These associations are mediated by intramembranal ionic attraction between a DAP12 aspartic acid residue and a lysine residue in the cognate receptor. The association between these receptors leads to either cellular activation or inhibition through dual tyrosine phosphorylation of the ITAM sequence by Src family kinases and further downstream signaling.
Development References (3)
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Hamerman JA, Ni M, Killebrew JR, Chu CL, Lowell CA. The expanding roles of ITAM adapters FcRgamma and DAP12 in myeloid cells.. Immunol Rev. 2009; 232(1):42-58. (Biology). View Reference
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Lanier LL, Corliss BC, Wu J, Leong C, Phillips JH. Immunoreceptor DAP12 bearing a tyrosine-based activation motif is involved in activating NK cells.. Nature. 1998; 391(6668):703-7. (Biology). View Reference
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Lanier LL. DAP10- and DAP12-associated receptors in innate immunity.. Immunol Rev. 2009; 227(1):150-60. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.