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Rat T/B/NK Cell Cocktail

Rat T/B/NK Cell Cocktail

(RUO)
Rat T/B/NK Cell Cocktail
Three color analysis of the expression of CD3, CD45RA and CD161a/NKR-P1A on rat splenocytes.  A single cell suspension of Lewis splenocytes was stained with either Isotype Control Cocktail - A (Cat. no. 558509, data not shown) or Rat T/B/NK Cocktail (Cat. no. 558495).  The figure on the left represents the CD3 and CD45RA profile gating on whole spleen.  The figure on the right represents the CD3 and CD161a/NKR-P1A profile gating on whole spleen.  Flow cytometry was performed on a BD FACSCalibur™ instrument.
Three color analysis of the expression of CD3, CD45RA and CD161a/NKR-P1A on rat splenocytes.  A single cell suspension of Lewis splenocytes was stained with either Isotype Control Cocktail - A (Cat. no. 558509, data not shown) or Rat T/B/NK Cocktail (Cat. no. 558495).  The figure on the left represents the CD3 and CD45RA profile gating on whole spleen.  The figure on the right represents the CD3 and CD161a/NKR-P1A profile gating on whole spleen.  Flow cytometry was performed on a BD FACSCalibur™ instrument.
Product Details
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BD Pharmingen™
Rat (QC Testing)
Flow cytometry (Routinely Tested)
RUO
AB_771203


Description

                                        Cocktail Component                Clone                Isotype

                                        APC anti-Rat CD3                        1F4                        mIgM

                                        FITC anti-Rat CD45RA                OX-33                mIgG1

                                        PE anti-Rat CD161a                10/78                mIgG1

The Rat T/B/NK Cocktail is a three-color reagent cocktail designed to identify rat T, B, and NK lymphocyte populations by direct immunofluorescent staining with flow cytometric analysis.  The monoclonal antibody clone 1F4 has been reported to react with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes and peripheral T lymphocytes.  The monoclonal antibody clone OX-33 has been reported to react with CD45RA found only on B lymphocytes.  The monoclonal antibody clone 10/78 has been reported to react with NKR-P1A (CD161a), 60-kDa homodimer expressed on all natural killer (NK) cells and a small subset of T lymphocytes.

This antibody cocktail is routinely tested by flow cytometric analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558495 Rev. 1
Citations & References
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Development References (3)

  1. Kraus E, Lambracht D, Wonigeit K, Hunig T. Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996; 26(11):2582-2586. (Biology). View Reference
  2. Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Biology). View Reference
  3. Woollett GR, Barclay AN, et al. Molecular and antigenic heterogeneity of the rat leukocyte common antigen from thymocytes and T and B lymphocytes. . Eur J Immunol. 1985; 15:168-173. (Biology).
558495 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.