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PE Mouse Anti-Human p16 Set

PE Mouse Anti-Human p16 Set

(RUO)
PE Mouse Anti-Human p16 Set
Flow cytometric analysis of p16 expression on WI-26 VA4 human lung cells. Permeabilized WI-26 VA4 cells were stained with either FITC Mouse Anti-Human p16 (Component No. 51-13384X; filled histogram) or FITC Mouse IgG1, κ Isotype Control (Component No. 51-13854X-6; open histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis was performed on a BD FACScan™.
Flow cytometric analysis of p16 expression on WI-26 VA4 human lung cells. Permeabilized WI-26 VA4 cells were stained with either FITC Mouse Anti-Human p16 (Component No. 51-13384X; filled histogram) or FITC Mouse IgG1, κ Isotype Control (Component No. 51-13854X-6; open histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis was performed on a BD FACScan™.
Product Details
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BD Pharmingen™
Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_396465


Description

Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. p16 inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16]. The gene, now also known as the CDKN2 gene, has been found to be mutated in a very high percentage of tumors, including 75% of melanoma cell lines.  Alternate names for p16 include p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

Flow cytometry:  For flow cytometry protocols, please refer to "Intracellular Flow" at our website: http://www.bdbiosciences.com/us/s/resources

Product Notices

  1. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556561 Rev. 3
Components
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Description Quantity/Size Part Number EntrezGene ID
PE Mouse Anti-Human p16 100 Tests (1 ea) 51-13385X N/A
PE Mouse IgG1, κ Isotype Control 100 Tests (1 ea) 51-13855X-4 N/A
556561 Rev. 3
Citations & References
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Development References (7)

  1. Kamb A, Gruis NA, Weaver-Feldhaus J, et al. A cell cycle regulator potentially involved in genesis of many tumor types. Science. 1994; 264(5157):436-440. (Biology). View Reference
  2. Li Y, Nichols MA, Shay JW, Xiong Y. Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. Cancer Res. 1994; 54(23):6078-6082. (Biology). View Reference
  3. Marx J. Link to hereditary melanoma brightens mood for p16 gene. Science. 1994; 265(5177):1364-1365. (Biology). View Reference
  4. Serrano M, Hannon GJ, Beach . A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature. 1993; 366(6456):704-707. (Immunogen). View Reference
  5. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines. Cancer Res. 1995; 55(3):505-509. (Biology). View Reference
  6. Tam SW, Shay JW, Pagano M. Differential expression and cell cycle regulation of the cyclin-dependent kinase 4 inhibitor p16Ink4. Cancer Res. 1994; 54(22):5816-5820. (Biology). View Reference
  7. Yeager T, Stadler W, Belair C, Puthenveettil J, Olopade O, Reznikoff C. Increased p16 levels correlate with pRb alterations in human urothelial cells. Cancer Res. 1995; 55(3):493-497. (Biology). View Reference
View All (7) View Less
556561 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.