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PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control

PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control

(RUO)
PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control

Cells grown in BD Mosaic™ hMSC SF Cell Culture Environment (Cat. No. 355700) were detached using BD™ Accutase™ Cell Detachment Solution (Cat. No 561527) and then stained with the PE hMSC Negative Cocktail and a cocktail of antibodies containing FITC CD90, PerCP-Cy5.5 CD105 and APC CD73 (See cat. No.562245) (solid lines) or the corresponding isotype control cocktails (dashed lines). The plots were derived from gated events based on light scattering characteristics of the MSCs. Cells were analyzed using a BD™ LSRII flow cytometry system.

Cells grown in BD Mosaic™ hMSC SF Cell Culture Environment (Cat. No. 355700) were detached using BD™ Accutase™ Cell Detachment Solution (Cat. No 561527) and then stained with the PE hMSC Negative Cocktail and a cocktail of antibodies containing FITC CD90, PerCP-Cy5.5 CD105 and APC CD73 (See cat. No.562245) (solid lines) or the corresponding isotype control cocktails (dashed lines). The plots were derived from gated events based on light scattering characteristics of the MSCs. Cells were analyzed using a BD™ LSRII flow cytometry system.

Product Details
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BD Stemflow™
Human (QC Testing)
Flow cytometry (Tested During Development)
RUO
AB_11153847


Description

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro. In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  This lineage set includes the set of ISCT negative markers as a cocktail and also a corresponding isotype control cocktail.

Description of Set Components

Vial                                                                                Contents                                                        Purpose        

PE hMSC Negative Cocktail                                        CD34 PE (Clone:581)                                Cocktail to identify potential contaminants

                                                                                CD11b PE (Clone: ICRF44)

                                                                                CD19 PE (Clone: HIB19)

                                                                                CD45 PE (Clone: HI30)

                                                                                HLA-DR PE (Clone: G46-6)

PE hMSC Isotype Control Negative Cocktail        mIgG1, κ PE (Clone: X40)                        Corresponding isotype control for PE hMSC

                                                                                mIgG2a, κ PE (Clone:G155-178)                Negative Cocktail

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
  2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562530 Rev. 1
Components
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Description Quantity/Size Part Number
PE hMSC Negative Cocktail 50 Tests (1 ea) 51-9007661
PE hMSC Isotype Control Negative Cocktail 50 Tests (1 ea) 51-9007662
562530 Rev. 1
Citations & References
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Development References (5)

  1. Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Biology: Flow cytometry). View Reference
  2. Dominici M, Le Blanc K, Mueller I, et. al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-317. (Biology: Flow cytometry). View Reference
  3. Horwitz EM, Le Blanc K, Dominici M, et. al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy. 2005; 7(5):393-395. (Biology). View Reference
  4. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-1301. (Biology: Flow cytometry). View Reference
  5. Sánchez L, Gutierrez-Aranda I, Ligero G, et al. Enrichment of Human ESC-Derived Multipotent Mesenchymal Stem Cells with Immunosuppressive and Anti-Inflammatory Properties Capable to Protect Against Experimental Inflammatory Bowel Disease. Stem Cells. 2010; 29(2):251-262. (Biology: Cell differentiation). View Reference
562530 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.