BD Stemflow™ Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit
(RUO)
Undifferentiated mouse ESC from the ES-E14TG2a cell line stained using the BD Stemflow Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit.
Undifferentiated mouse ESC from the ES-E14TG2a cell line stained using the BD Stemflow Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit.
Product Details
BD Stemflow™
Mouse (QC Testing), Rhesus (Reported)
RUO
AB_2869357
Description
Preparation And Storage
The entire BD Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit must be stored in the dark at 2° to 8°C. Do not freeze.
560585 Rev. 1
Components
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| PE Mouse anti-Mouse Nanog | 1.5 mL (1 ea) | 51-9006405 | N/A |
| PE Mouse IgG1, κ Isotype Control | N/A | 51-9006406 | N/A |
| PerCP-Cy™5.5 Mouse anti-Oct3/4 | 1.5 mL (1 ea) | 51-9006267 | N/A |
| PerCP-Cy™5.5 Mouse IgG1, κ Isotype Control | N/A | 51-9006272 | N/A |
| Alexa Fluor® 647 Mouse anti-Sox2 | 1.5 mL (1 ea) | 51-9006407 | N/A |
| Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control | N/A | 51-9006410 | N/A |
| Anti-Mouse Ig, κ | 6 mL (1 ea) | 51-9006274 | N/A |
| Negative Control (PBS with 1% BSA) | 50 Tests (1 ea) | 51-9006227 | N/A |
| Perm/Wash Buffer | N/A | 51-9006275 | N/A |
| Fixation Buffer | 50 mL (1 ea) | 51-9006276 | N/A |
560585 Rev. 1
Citations & References
Development References (8)
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Boiani M, Schöler HR. Regulatory networks in embryo-derived pluripotent stem cells. Nat Rev Mol Cell Biol. 2005; 6:872–881. (Biology).
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Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 2003; 113:643-655. (Biology). View Reference
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Kim J, Chu J, Shen X, Wang J, Orkin SH. An extended transcriptional network for pluripotency of embryonic stem cells. Cell. 2008; 312:1049–1061. (Biology).
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Loh Y, Wu Q, Chew J, et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nature Genetics. 2006; 38:431–440. (Biology).
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Masui S, Nakatake Y, Toyooka,Y, et al. Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nature Cell Biology. 2007; 9:625–635. (Biology).
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Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990; 60(3):461-472. (Biology). View Reference
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Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
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Yamanaka, S. Pluripotency and nuclear reprogramming. Philos Trans R Soc Lond B Biol Sci. 2008; 363(1500):2079-2087. (Biology). View Reference
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Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
