Description
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Preparation And Storage
The entire BD Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit must be stored in the dark at 2–8°C. Do not freeze.
Recommended Assay Procedures
An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells).
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| FITC Rat anti-Mouse CD34 | 0.2 mL (1 ea) | 51-9006306 | N/A |
| PE Anti-Mouse c-Kit | 0.2 mL (1 ea) | 51-9006307 | N/A |
| PE-Cy™7 Anti-Mouse Sca-1 | 0.2 mL (1 ea) | 51-9006308 | N/A |
| APC Mouse Lineage Antibody Cocktail | N/A | 51-9006309 | N/A |
| FITC Rat IgG2a, κ Isotype Control | N/A | 51-9006310 | N/A |
| PE Rat IgG2b, κ Isotype Control | N/A | 51-9006312 | N/A |
| PE-CY™7 Rat IgG2a, κ Isotype Control | N/A | 51-9006313 | N/A |
| APC Mouse Lineage Isotype Control Cocktail | N/A | 51-9006314 | N/A |
| Purified Rat anti-Mouse CD16/CD32 (Mouse BD Fc Block™) | N/A | 51-9006315 | N/A |
| 7-AAD | N/A | 51-9006316 | N/A |
| Negative Control | 6 mL (1 ea) | 51-90-9001291 | 500183 |
| Anti-Rat and Anti-Hamster Ig, κ | 6 mL (1 ea) | 51-90-9000949 | 500183 |
Development References (5)
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Ema H, Morita Y, Yamazaki S, et al. Adult mouse hematopoietic stem cells: purification and single-cell assays. Nat Protoc. 2006; 1:2979–2987. (Biology).
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Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med. 1996; 183:1797–1806. (Biology).
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Ikuta K, Weissman IL. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proc Natl Acad Sci U S A. 1992; 89(4):1502-1506. (Biology). View Reference
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Mishell BB, Shiigi SM, Henrcy C, et al. Mishell BB, Shiigi SM, ed. Selected Methods in Cellular Immunology. San Francisco: WH Freeman and Company; :11-12.
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Ploemacher RE, van der Sluijs JP, Voerman JS, Brons NH. An in vitro limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse. Blood. 1989; 74:2755–2763. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
