PerCP-Cy™5.5 Mouse Anti-Human CD41a

BD™ PerCP-Cy™5.5 Mouse Anti-Human CD41a

Name: PerCP-Cy™5.5 Mouse Anti-Human CD41a
Brand: BD™
SKU: 340931

Clone HIP8

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: ITGA2B; Integrin alpha-2b (αIIb); Platelet glycoprotein IIb (GPIIb)

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Purified platelet membrane glycoproteins

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

Workshop Number: IV P38

Entrez Gene ID: 3674

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340931 Rev. 1

Antibody Details

HIP8

The CD41a antibody, clone HIP8, is derived from the hybridization of P3X63 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with purified platelet membrane glycoproteins.

The CD41a antibody recognizes the calcium-dependent gpIIb/IIIa complex, with a molecular weight of 140 kilodaltons (kDa).

340931 Rev. 1

Format Details

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

340931 Rev.1

Citations & References

Development References (12)

Bettaieb A, Fromont P, Louache F, et al. Presence of cross-reactive antibody between human immunodeﬁciency virus (HIV) and platelet glycoproteins in HIV-related immune thrombocytopenic purpura. Blood. 1992; 80:162-169. (Biology).
Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
Chen CS, Thiagarajan P, Schwartz SM, Harlan JM, Heimark RL. The platelet glycoprotein IIb/IIIa-like protein in human endothelial cells, promotes adhesion but not initial attachment to extracellular matrix. J Cell Biol. 1987; 105:1885-1892. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Feng R, Shimazaki C, Inaba T, et al. CD34+/CD41a+ cells best predict platelet recovery after autologous peripheral blood stem cell transplantation. Bone Marrow Transplant. 1998; 21:1217-1222. (Biology).
Jennings LK, Ashmun RA, Wang WC, Docter ME. Analysis of human platelet glycoproteins IIb-IIIa and Glanzmann’s thrombasthenia in whole blood by ﬂow cytometry. Blood. 1986; 68:173-179. (Biology).
Kitagawa M, Yoshida S, Ishige I, et al. Immunolocalization of platelet-derived growth factor, transforming growth factor-β, and ﬁbronectin in acute megakaryoblastic leukemia manifesting tumor formation. Human Pathol. 1994; 25:723-726. (Biology).
Phillips DR, Charo IF, Parise LV, Fitzgerald LA. The platelet membrane glycoprotein IIb-IIIa complex. Blood. 1988; 71:831-843. (Biology).
Spangenberg P, Redlich H, Bergmann I, Lösche W, Götzrath M, Kehrel B. The platelet glycoprotein IIb/IIIa complex is involved in the adhesion of activated platelets to leukocytes. Thromb Haemos. 1993; 70:514-521. (Biology).
Yasunaga M, Ryo R, Konaka Y, et al. Extramedullary tumor as presentation of leukemia: establishment of a new human gpIIb- and gpIIIa-positive leukemia cell line. Ann Hematol. 1994; 68:145-151. (Biology).
de Haas M, von dem Borne A. CD41/CD61 Workshop Panel report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:643-645.
von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.

View All (12)

340931 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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