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PE Mouse Anti-Human CD38
Product Details
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BD Quantibrite™
T10; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; OKT10
Human
Mouse IgG1, κ
Human BJAB B cell line
Flow cytometry
12.5 μg/mL
20 μL
III B918
952
AB_400453
Phosphate buffered saline with BSA and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The QuantiBRITE CD38 PE (≥95% 1:1 PE:mAb ratio) reagent is supplied as 12.5 μg in 1 mL (12.5 μg/mL) of PBS containing bovine serum albumin and 0.1% sodium azide. Vials should be stored at 2–8°C. QuantiBRITE reagents should not be frozen and should be protected from prolonged exposure to light. The reagent is stable for the period shown on the bottle label when stored as directed.

342371 Rev. 1
Antibody Details
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HB7

The CD38 antibody, clone HB7, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the BJAB cell line.

The CD38 antibody recognizes an integral membrane glycoprotein of 45 kilodaltons (kDa), with a protein core of 35 kDa. The CD38 antigen is also known as T10, ADP-ribosyl cyclase, and cyclic ADP ribose hydrolase 1.

342371 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
342371 Rev.1
Citations & References
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Development References (25)

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  14. Otawa M, Kawanishi Y, Iwase O, Shoji N, Miyazawa K, Ohyashiki K. Comparative multi-color flow cytometric analysis of cell surface antigens in bone marrow hematopoietic progenitors between refractory anemia and aplastic anemia. Leuk Res. 2000; 24(4):359-366. (Biology).
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  17. Rawstron AC, Owen RG, Davies FE, et al. Circulating plasma cells in multiple myeloma: characterization and correlation with disease stage. Br J Haematol. 1997; 97:46-55. (Biology).
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  20. Shinjo K, Takeshita A, Higuchi M, Ohnishi K, Ohno R. Erythropoietin receptor expression on human bone marrow erythroid precursor cells by a newly-devised quantitative flow-cytometric assay. Br J Haematol. 1997; 96(3):551-558. (Biology).
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342371 Rev. 1

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