FITC Mouse Anti-Human CD15

BD™ FITC Mouse Anti-Human CD15

Name: FITC Mouse Anti-Human CD15
Brand: BD™
SKU: 347423

Clone MMA

(RUO (GMP))

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Product Details

Brand: BD™

Reactivity: Human

Isotype: Mouse BALB/c IgM, κ

Immunogen: U-937 histiocytic cell line

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 20 μL

Entrez Gene ID: 2526

RRID: AB_400299

Storage Buffer: HEPES buffer with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

MMA

The CD15 antibody, clone MMA, is derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the U-937 histiocytic cell line.

CD15 recognizes a human myelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.

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Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

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Citations & References

Development References (4)

Hanjan SN, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982 May; 23(2):172-188. (Biology). View Reference
Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984; 82:29. (Biology).
Pinkus GS, Thomas P, Said JW. Leu-M1—A marker for Reed-Sternberg cells in Hodgkin's disease: an immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985; 119:244. (Biology).
Skubitz K, Balke J, Ball E, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:800-805.

View All (4)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.