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      Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5

      BD Oncomark™ Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5

      (RUO (GMP))
      Product Details
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      BD Oncomark™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with gelatin and 0.1% sodium azide.


      Description

      Clone FMC7, is generated from the fusion of P3-NS1-1-AG4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human B-lymphoblastoid cell line HRIK.

      The CD23 antibody, clone EBVCS-5, is derived from hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with in vitro– transformed EBV cell line.

      The CD19 antibody, clone SJ25C1, is derived from hybridization of Sp2/0 mouse cells with spleen cells from BALB/c mice immunized with NALM1 + NALM16 cells.

      The FCM7 antibody recognizes a 105-kilodalton (kDa) membrane glycoprotein expressed on a subset of B lymphocytes.

      The CD23 antibody recognizes a human B-lymphocyte differentiation antigen, with a molecular weight of 45 kDa, that is the low-affinity Fc epsilon receptor.

      The CD19 (SJ25C1) antibody recognizes a 90-kDa antigen that is present on human B lymphocytes.

      Preparation And Storage

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      341148 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      FMC7 FITC FMC7 IgM, N/A
      CD23 PE EBVCS-5 IgG1, κ N/A
      CD19 PerCP-Cy5.5 SJ25C1 IgG1, κ 930
      341148 Rev. 1
      Citations & References
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      Development References (32)

      1. Almasri NM, Duque RE, Iturraspe J, Everett E, Braylan RC. Reduced expression of CD20 antigen as a characteristic marker for chronic lymphocytic leukemia. Am J Hematol. 1992; 40:259-263. (Biology).
      2. Almasri NM, Iturraspe JA, Braylan RC. CD10 expression in follicular lymphoma and large cell lymphoma is different from that of reactive lymph node follicles. Arch Pathol Lab Med. 1998; 122:539-544. (Biology).
      3. Bloem AC, Chand MA, Dollekamp I, Rijkers GT. Functional properties of human B cell subpopulations defined by monoclonal antibodies HB4 and FMC7. J Immunol. 1988; 140:768-773. (Biology).
      4. Brooks DA, Beckman IG, Bradley J, McNamara PJ, Thomas ME, Zola H. Human lymphocyte markers defined by antibodies derived from somatic cell hybrids, IV, A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes. J Immunol. 1981; 126:1373-1377. (Biology).
      5. Capron M, Jouault T, Prin L, et al. Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages.. J Exp Med. 1986; 164(1):72-89. (Biology). View Reference
      6. DiGiuseppe JA, Borowitz MJ. Clinical utility of flow cytometry in the chronic lymphoid leukemias. Semin Immunol. 1998; 25(1):6-10. (Biology). View Reference
      7. Geisler CH, Larsen JK, E HN, et al. Prognostic importance of flow cytometric immunophenotyping of 540 consecutive patients with B-cell chronic lymphocytic leukemia. Blood. 1991; 78:1795-1802. (Biology).
      8. Gordon J, Rowe M, Walker L, Guy G. Ligation of the CD23,p45 (Blast-2, EBVCS) antigen triggers the cell-cycle progression of activated B lymphocytes. Eur J Immunol. 1986; 16:1075-1080. (Biology).
      9. Gordon J, Webb A, Walker L, Guy G, Rowe M. Evidence of an association between CD23 and the receptor for a low molecular weight B-cell growth factor. Eur J Immunol. 1986; 16:1627. (Biology).
      10. Hassan IB, Hagberg H, Sundström C. Immunophenotype of hairy-cell leukemia. Eur J Haematol. 1990; 45:172-176. (Biology).
      11. Hoffkes HG, Schmidtke G, Uppenkamp M, Schmucker U. Multiparametric immunophenotyping of B cells in peripheral blood of healthy adults by flow cytometry. Clin Diagn Lab Immunol. 1996; 3:30-36. (Biology).
      12. Huh YO, Pugh WC, Kantarjian HM, et al. Detection of subgroups of chronic B-cell leukemias by FMC7 monoclonal antibody. Am J Clin Pathol. 1994; 101:283-289. (Biology).
      13. Hübl W, Iturraspe J, Braylan RC. FMC7 antigen expression on normal and malignant B-cells can be predicted by expression of CD20. Cytometry. 1998; 34:71-74. (Biology).
      14. Kikutani H, Inui S, Sato R, et al. Molecular structure of human lymphocyte receptor for immunoglobulin E.. Cell. 1986; 47(5):657-65. (Biology). View Reference
      15. Kikutani H, Suemura M, Owaki H, et al. Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching.. J Exp Med. 1986; 164(5):1455-69. (Biology). View Reference
      16. Kilo MN, Dorfman DM. The utility of flow cytometric immunophenotypic analysis in the distinction of small lymphocytic lymphoma/chronic lymphocytic leukemia from mantle cell lymphoma. Am J Clin Pathol . 1996; 105:451-457. (Biology).
      17. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
      18. Lucio P, Parreira A, van den Beemd MW, et al. Flow cytometric analysis of normal B cell differentiation: a frame of reference for the detection of minimal residual disease in precursor-B- ALL. Leukemia. 1999; 13(3):419-427. (Biology).
      19. Marti GE, Zenger V, Caproaso NE, et al. Antigenic expression of B-cell chronic lymphocytic leukemic lymphocytes. Anal Quant Cytol Histol. 1989; 11:315-323. (Biology).
      20. Matutes E, Morilla R, Owusu-Ankomah K, Houliham A, Meeus P, Catovsky D. The immunophenotype of hairy cell leukemia (HCL). Proposal for a scoring system to distinguish HCL from B-cell disorders with hairy or villous lymphocytes. Leuk Lymphoma. 1994; 14:57-61. (Biology).
      21. Matutes E, Owusu-Ankomah K, Morilla R, et al. The immunological profile of B-cell disorders and proposal of a scoring system for the diagnosis of CLL. Leukemia. 1994; 8(10):1640-1645. (Biology).
      22. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
      23. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
      24. Nadler LM. Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York, NY: Springer-Verlag; 1986:25-26.
      25. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
      26. Singleton TP, Anderson MM, Ross CW, Schnitzer B. Leukemic phase of mantle cell lymphoma, blastoid variant. Am J Clin Pathol. 1999; 111:495-500. (Biology).
      27. Tedder TF, Zhou LJ, Engel P. The CD19/CD21 signal transduction complex of B lymphocytes. Immunol Today. 1994; 15(9):437-442. (Biology). View Reference
      28. Thorley-Lawson D, Nadler L, Bhan A, Schooley R. Blast-2 (EBVCS), an early cell surface marker of human B cell activation, is superinduced by Epstein Barr Virus. J Immunol. 1985; 134:3007-3012. (Biology).
      29. Tworek JA, Singleton TP, Schnitzer B, Hsi ED, Ross CW. Flow cytometric and immunohistochemical analysis of small lymphocytic lymphoma, mantle cell lymphoma, and plasmacytoid small lymphocytic lymphoma. Am J Clin Pathol. 1998; 110:582-589. (Biology).
      30. Yukawa K, Kikutani H, Owaki H, et al. A B-cell– specific differentiation antigen, CD23, is a receptor for IgE (FcεR) on lymphocytes. J Immunol. 1987; 138:2576-2580. (Biology).
      31. Zola H, Neoh SH, Potter A, Melo JV, De Oliveria MSP, Catovsky D. Markers of differentiated B cell leukaemia: CD22 antibodies and FMC7 react with different molecules. Disease Markers. 1987; 5:227-235. (Biology).
      32. van Wering E, Beishuizen A, Roeffen E. Immunophenotypic changes between diagnosis and relapse in childhood acute lymphoblastic leukemia. Leukemia. 1995; 9:1523-1533. (Biology).
      View All (32) View Less
      341148 Rev. 1

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      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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