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      Anti-Human CD8 FITC/CD56 PE/CD3 PerCP-Cy™5.5

      BD Oncomark™ Anti-Human CD8 FITC/CD56 PE/CD3 PerCP-Cy™5.5

      (RUO (GMP))
      Product Details
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      BD Oncomark™
      Human
      Flow cytometry
      RUO (GMP)
      Buffered saline with BSA and 0.1% sodium azide.


      Description

      CD8, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

      CD56, clone NCAM16.2, is derived from the hybridization of mouse P3X-63-Ag8.653 cells with lymph nodes from BALB/c mice immunized with immunoaffinity-enriched adult human brain NCAM.

      CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

      The CD8 antigen is expressed on the 32-kilodalton (kd) α subunit of a disulfide-linked bimolecular complex.The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (Mr 32, 30 kd), while CD8+CD16+ NK lymphocytes and CD8+ T-cell receptor (TCR)- γ/δ+ T lymphocytes express an α/α homodimer (Mr 30 kd). CD8+ TCR- α/β + T lymphocytes can express either an α/α homodimer or an α/β heterodimer.

      CD56 (NCAM16.2) recognizes an extracellular immunoglobulin-like domain common to three molecular weight forms (Mr 120, 140, and 180 kd) of the neural cell adhesion molecule (NCAM).

      CD3 reacts with the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).

      Preparation And Storage

      Store vials at 2° to 8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      341142 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD8 FITC SK1 IgG1, κ N/A
      CD56 (NCAM-1) PE NCAM16.2 IgG2b, κ N/A
      CD3 PerCP-Cy5.5 SK7 IgG1, κ N/A
      341142 Rev. 1
      Citations & References
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      Development References (27)

      1. Brenner MB, McClean J, Dialynas DP, et al. Identification of a putative second T cell receptor. Nature. 1986; 322:145-49. (Biology).
      2. Chan WC, Gu LB, Masih A, et al. Large granular lymphocyte proliferation with the natural killer-cell phenotype. Am J Clin Pathol. 1992; 97:353-358. (Biology).
      3. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
      4. Cunningham BA, Hemperly JJ, Murray BA, Prediger EA, Brackenbury R, Edelman GM. Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. Science. 1987; 236(4803):799-806. (Biology). View Reference
      5. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
      6. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
      7. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
      8. Gentile TC, Uner AH, Hutchison RE, et al. CD3+, CD56+ aggressive variant of large granular lymphocyte leukemia. Blood. 1994; 84(7):2315-2321. (Biology).
      9. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
      10. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
      11. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
      12. Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
      13. Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH. Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991; 146(12):4421-4426. (Biology). View Reference
      14. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). View Reference
      15. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). View Reference
      16. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
      17. Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
      18. Loughran TP, Jr. Clonal diseases of large granular lymphocytes. Blood. 1993; 82:43844. (Biology).
      19. Macon WR, Williams ME, Greer JP, et al. Natural killer-like T-cell lymphomas: aggressive lymphomas of T-large granular lymphocytes. Blood. 1996; 87:1474-1483. (Biology).
      20. Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
      21. Okuno SH, Tefferi A, Hanson C, Katzmann JA, Li CY, Witzig TE. Spectrum of diseases associated with increased proportions or absolute numbers of peripheral blood natural killer cells. Br J Haematol. 1996; 93:810-812. (Biology).
      22. Ortaldo JR, Winkler-Pickett RT, Yagita H, Young HA. Comparative studies of CD3– and CD3+ CD56+ cells: examination of morphology, functions, T cell receptor rearrangement, and pore-forming protein expression. Cell Immunol. 1991; 136:486-495. (Biology).
      23. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
      24. Ritz J, Schmidt RE, Michon J, Hercend T, Schlossman SF. Characterization of functional surface structures on human natural killer cells. Adv Immunol. 1988; 42:181-211. (Biology).
      25. Schubert J, Lanier LL, Schmidt RE. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:699-702.
      26. Terry LA, DiSanto JP, Small TN, Flomenberg N. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:345-346.
      27. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
      View All (27) View Less
      341142 Rev. 1

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      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

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