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Anti-Human CD4 FITC/CD25 PE/CD3 PerCP-Cy™5.5

BD Oncomark™ Anti-Human CD4 FITC/CD25 PE/CD3 PerCP-Cy™5.5

(RUO (GMP))
Product Details
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BD Oncomark™
Human
Flow cytometry
RUO (GMP)
Buffered saline with BSA and 0.1% sodium azide.


Description

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.

CD25, clone 2A3, is derived from hybridization of mouse myeloma cell line NS-1 myeloma cells with spleen cells from BALB/c mice immunized with PHA-activated human T lymphocytes.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

The CD4 antigen, Mr 55 kilodalton (kd), is present on T-helper/inducer lymphocytes and monocytes.

CD25 recognizes an antigen that is the human low-affinity interleukin-2 receptor (IL-2R). The antigen has a molecular weight of 55 kd.

CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).

Preparation And Storage

Store vials at 2° to 8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341134 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD4 FITC SK3 IgG1, κ 920
CD3 PerCP-Cy5.5 SK7 IgG1, κ N/A
CD25 (IL-2 Receptor α) 2A3 IgG1, κ N/A
341134 Rev. 1
Citations & References
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Development References (25)

  1. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories: T2 protocol. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies : specification, classification, nomenclature = Typage leucocytaire : antigènes de différenciation leucocytaire humains révélés par les anticorps monoclonaux : "Rapports des études communes". Berlin New York: Springer-Verlag; 1984:25-60.
  2. Buckle GJ, Hafler DA, Hollsberg P. HTLV-I-induced T-cell activation. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1996; 13:S107-S113. (Biology).
  3. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  4. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
  5. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  6. Ferrara F, Cimino R, Antinolfi I, et al. Clinical relevance of immunological dissection in T-ALL: a report on 20 cases with stem cell (CD7+, CD4-, CD8-, CD1-) phenotype. Am J Hematol. 1992; 40:98-102. (Biology).
  7. Freedman AS, Nadler LM. Immunologic markers in non-Hodgkin's lymphoma. Hematol Oncol Clin North Am. 1991; 5(5):871-889. (Biology).
  8. Ginaldi L, Matutes E, Farahat N, De Martinis M, Morilla R, Catovsky D. Differential expression of CD3 and CD7 in T-cell malignancies: a quantitative study by flow cytometry. Br J Haematol. 1996; 93:921-927. (Biology).
  9. Hastrup N, Ralfkiaer E, Pallesen G. Aberrant phenotypes in peripheral T cell lymphomas. J Clin Pathol. 1989; 42:398-402. (Biology).
  10. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  11. Hemler ME, Brenner MB, McLean JM, Strominger JL. Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells. Proc Natl Acad Sci USA. 1984; 81:2172-2175. (Biology).
  12. Honda M, Yamamoto N, Nagao S, Tokunaga T. High Levels of soluble interleukin 2 receptors released from human retrovirus-infected cells. Jpn J Cancer Res (Gann). 1987; 78:889-893. (Biology).
  13. Jackson AL, Matsumoto H, Janszen M, Maino V, Blidy A, Shye S. Restricted expression of p55 interleukin 2 receptor (CD25) on normal T cells. Clin Immunol Immunopathol. 1990; 54(1):126-133. (Biology). View Reference
  14. Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). View Reference
  15. Knowles DM. Immunophenotypic and antigen receptor gene rearrangement analysis in T cell neoplasia. Am J Pathol. 1989; 134:761-785. (Biology).
  16. Knowles R. Reinherz E, Haynes B, Nadler L, Bernstein I, ed. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.
  17. Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
  18. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  19. Nagatani T, Miyazawa M, Matsuzaki T, et al. Comparative study of cutaneous T-cell lymphoma and adult T-cell leukemia/lymphoma. Semin Dermatol. 1994; 13:216-222. (Biology).
  20. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  21. Robb RJ, Greene WC, Rusk CM. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen. J Exp Med. 1984; 160(4):1126-1146. (Biology). View Reference
  22. Schwarting R, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:399-402.
  23. Shahabuddin S. Expression and release of IL-2 receptor and production of IL-2 by activated T lymphocyte subsets. J Clin Lab Immunol. 1991; 36:27-32. (Biology).
  24. Teshigawara K, Wang HM, Kato K, Smith KA. Interleukin 2 high-affinity receptor expression requires two distinct binding proteins. J Exp Med. 1987; 165(1):223-238. (Biology). View Reference
  25. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (25) View Less
341134 Rev. 1

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