Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- CF™ is a trademark of Biotium, Inc.
Companion Products
The 22D1 monoclonal antibody specifically recognizes mouse CD209 antigen-like protein B (CD209b) which is also known as Mouse SIGN-related 1 (mSIGNR1 or SIGN-R1). CD209b is a mouse homolog of human CD209 (DC-SIGN) and is also referred to as DC-SIGN-related protein 1 (DC-SIGNR1). CD209b is an ~37 kDa type II transmembrane glycoprotein that is encoded by Cd209b (CD209b antigen) which belongs to the C-type lectin family. It contains a single C-terminal C-type lectin domain and is involved in the recognition and internalization of microbial polysaccharides. CD209b is expressed on macrophages residing in the splenic marginal zone, the medullary and trabecular sinuses of lymph nodes, and peritoneal cavity. CD209b is involved in the clearance and innate immune responses to microbial molecules including polysaccharides and lipopolysaccharides (LPS) and the uptake of encapsulated microbes. It may also affect leucocyte migration through its adhesive interaction with CD54 (ICAM-2).
Development References (4)
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Camara A, Lavanant AC, Abe J, et al. CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling.. Proc Natl Acad Sci U S A. 2022; 119(3):e2108540119. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
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Kang YS, Kim JY, Bruening SA, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.. Proc Natl Acad Sci USA. 2004; 101(1):215-20. (Immunogen: Flow cytometry, Fluorescence microscopy, In vivo exacerbation, Western blot). View Reference
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Kawauchi Y, Igarashi M, Kojima N. C-type lectin receptor SIGNR1 expressed on peritoneal phagocytic cells with an immature dendritic cell-like phenotype is involved in uptake of oligomannose-coated liposomes and subsequent cell maturation.. Cell Immunol. 2014; 287(2):121-8. (Clone-specific: Flow cytometry). View Reference
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Taylor PR, Martinez-Pomares L, Stacey M, Lin HH, Brown GD, Gordon S. Macrophage receptors and immune recognition.. Annu Rev Immunol. 2005; 23:901-44. (Clone-specific: In vivo exacerbation). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.