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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The BAH-1 monoclonal antibody was raised against human megakaryocytic cells. It was originally proposed that clone BAH-1 was specific for human CD110, also known as c-Mpl or TPO-R. Data has emerged to suggest that BAH-1 antibody may not recognize CD110. However, there is strong evidence that it recognizes an antigen that is highly expressed on human Megakaryocyte-Erythroid Progenitor (MEP) cells. In combination with CD71, BAH-1 expression has been used to distinguish subsets of Hematopoietic Stem Cells and Myeloid Progenitors in human fetal liver, cord blood, and adult bone marrow. BAH-1 antibody can induce megakaryocytopoiesis of both mouse and human bone marrow cells in vitro.
Development References (4)
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Abbott C, Huang G, Ellison AR, et al. Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applications.. Hybridoma (Larchmt). 2010; 29(2):103-13. (Clone-specific: Flow cytometry). View Reference
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Deng B, Banu N, Malloy B, et al. An agonist murine monoclonal antibody to the human c-Mpl receptor stimulates megakaryocytopoiesis. Blood. 1998; 92(6):1981-1988. (Immunogen: Cell differentiation, (Co)-stimulation, Flow cytometry). View Reference
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Edvardsson L, Dykes J, Olofsson T. Isolation and characterization of human myeloid progenitor populations--TpoR as discriminator between common myeloid and megakaryocyte/erythroid progenitors.. Exp Hematol. 2006; 34(5):599-609. (Clone-specific: Flow cytometry). View Reference
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Notta F, Zandi S, Takayama N, et al. Distinct routes of lineage development reshape the human blood hierarchy across ontogeny.. Science. 2016; 351(6269):aab2116. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.