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      RB780 Rat Anti-Mouse CD38
      RB780 Rat Anti-Mouse CD38
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB780 Rat Anti-Mouse CD38 antibody (Cat. No. 755623; Right Plot) on viable C57BL/6 mouse splenocytes, with Isotype Control (Cat. No. 568698; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      RB780 Rat Anti-Mouse CD38
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB780 Rat Anti-Mouse CD38 antibody (Cat. No. 755623; Right Plot) on viable C57BL/6 mouse splenocytes, with Isotype Control (Cat. No. 568698; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      Product Details
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      BD OptiBuild™
      ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; I-19; NIM-R5
      Mouse (Tested in Development)
      Rat IgG2a, κ
      Mouse Bone Marrow Pre-B cells
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      2. Researchers should determine the optimal concentration of this reagent for their individual applications.
      3. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      6. An isotype control should be used at the same concentration as the antibody of interest.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      10. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
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      755623 Rev. 2
      Antibody Details
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      90/CD38

      The 90 monoclonal antibody specifically binds to CD38, a 42 kDa transmembrane glycoprotein on immature and mature, resting and activated, B lymphocytes. In contrast to humans, CD38 expression is down-regulated on mouse germinal center B cells and plasma cells. CD38 is also expressed on a subpopulation of thymic and peripheral T cells, NK cells, and splenic macrophages. Furthermore, CD38 has been detected on bone marrow-derived hematopoietic stem cells. The CD38 molecule is reported to exhibit both cyclase and hydrolase activities and plays a role in lymphocyte activation. CD31, both human and mouse, is reported to be a ligand for human CD38.

      755623 Rev. 2
      Format Details
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      RB780
      The BD Horizon RealBlue™ 780 (RB780) Dye is part of the BD family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 781-nm. Driven by BD innovation, RB780 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB780 can be used as an alternative to PE-Cy7 and we recommend using an optical filter centered near 780-nm (eg, a 780/60-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PE-Cy7. RB780 is on average brighter than PE-Cy7 and has minimal spillover into Yellow-Green detectors.
      altImg
      RB780
      Blue 488 nm
      498 nm
      781 nm
      755623 Rev.2
      Citations & References
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      View product citations for antibody "755623" on CiteAb

      Development References (11)

      1. BD Biosciences Pharmingen. Unpublished results. .
      2. Bean AG, Godfrey DI, Ferlin WG, et al. CD38 expression on mouse T cells: CD38 defines functionally distinct subsets of alpha beta TCR+CD4-CD8- thymocytes. Int Immunol. 1995; 7(2):213-221. (Biology). View Reference
      3. Cockayne DA, Muchamuel T, Grimaldi JC, et al. Mice deficient for the ecto-nicotinamide adenine dinucleotide glycohydrolase CD38 exhibit altered humoral immune responses. Blood. 1998; 92(4):1324-1333. (Biology). View Reference
      4. Deaglio S, Morra M, Mallone R, et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J Immunol. 1998; 160(1):395-402. (Biology). View Reference
      5. Erickson LD, Vogel LA, Cascalho M, et al. B cell immunopoiesis: visualizing the impact of CD40 engagement on the course of T cell-independent immune responses in an Ig transgenic system. Eur J Immunol. 2000; 30(11):3121-3131. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
      6. Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998; 330(3):1129-1135. (Biology). View Reference
      7. Howard M, Grimaldi JC, Bazan JF, et al. Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38. Science. 1993; 262(5136):1056-1059. (Biology). View Reference
      8. Lund F, Solvason N, Grimaldi JC, Parkhouse RM, Howard M. Murine CD38: an immunoregulatory ectoenzyme. Immunol Today. 1995; 16(10):469-473. (Biology). View Reference
      9. Oliver AM, Martin F, Kearney JF. Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997; 158(3):1108-1115. (Immunogen: ELISA, Flow cytometry, Fluorescence activated cell sorting, IC/FCM Block, Immunofluorescence, Immunoprecipitation). View Reference
      10. Oliver AM. Personal Communication. .
      11. Randall TD, Lund FE, Howard MC, Weissman IL. Expression of murine CD38 defines a population of long-term reconstituting hematopoietic stem cells. Blood. 1996; 87(10):4057-4067. (Biology). View Reference
      View All (11) View Less
      755623 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.