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      RB705 Mouse Anti-Human TLR4 (CD284)
      RB705 Mouse Anti-Human TLR4 (CD284)
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB705 Mouse Anti-Human TLR4 (CD284) antibody (Cat. No. 757134; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570261; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
      RB705 Mouse Anti-Human TLR4 (CD284)
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB705 Mouse Anti-Human TLR4 (CD284) antibody (Cat. No. 757134; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570261; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
      RB705 Mouse Anti-Human TLR4 (CD284) RB705 Mouse Anti-Human TLR4 (CD284)
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB705 Mouse Anti-Human TLR4 (CD284) antibody (Cat. No. 757134; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570261; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB705 Mouse Anti-Human TLR4 (CD284) antibody (Cat. No. 757134; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570261; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
      Product Details
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      BD OptiBuild™
      TLR4; CD284; Toll-like Receptor 4; ARMD10
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human TLR4 Transfected Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      7099
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
      12. For U.S. patents that may apply, see bd.com/patents.
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      757134 Rev. 1
      Antibody Details
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      TF901

      The TF901 monoclonal antibody specifically binds to TLR4 (Toll-like receptor 4) which is also known as CD284. TLR4 is a 110 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor (TLR) family. TLR4 is expressed on monocytes, macrophages, granulocytes, dendritic cells and endothelial cells. TLR4 binds lipopolysaccharide (LPS) which is a major membrane component of Gram-negative bacteria. It thus acts as an innate immune recognition receptor against many pathogens. In association with MD-2, LPS binds to and signals through TLR4 receptors by MyD88-dependent and MyD88-independent pathways to induce the cellular production of proinflammatory cytokines. TF901 antibody binding is not influenced by the human TLR4 D299G/T399I polymorphism. Preincubation of TLR4-positive cells with the TF901 antibody blocks the subsequent binding of the HTA125 antibody which is also specific for human TLR4.

      757134 Rev. 1
      Format Details
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      RB705
      The BD Horizon RealBlue™ 705 (RB705) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 707-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB705 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB705 can be used as an alternative to PerCP-Cy5.5 or BB700 and we recommend using an optical filter centered near 710-nm (e.g., a 695/40 or 710/50-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PerCP-Cy5.5. RB705 is on average brighter than PerCP-Cy5.5 and BB700, and has minimal spillover into Yellow-Green detectors.
      altImg
      RB705
      Blue 488 nm
      498 nm
      707 nm
      757134 Rev.1
      Citations & References
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      View product citations for antibody "757134" on CiteAb

      Development References (7)

      1. Akashi S, Ogata H, Kirikae F, et al. Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2. Biochem Biophys Res Commun. 2000; 268(1):172-177. (Biology). View Reference
      2. Akira S. Toll-like receptors and innate immunity. Adv Immunol. 2001; 78:1-56. (Biology). View Reference
      3. Beutler B. Tlr4: central component of the sole mammalian LPS sensor. Curr Opin Immunol. 2000; 12(1):20-26. (Biology). View Reference
      4. Rock FL, Hardiman G, Timans JC, Kastelein RA, Bazan JF. A family of human receptors structurally related to Drosophila Toll. Proc Natl Acad Sci U S A. 1998; 95(2):588-593. (Biology). View Reference
      5. Shimazu R, Akashi S, Ogata H, et al. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 1999; 189(11):1777-1782. (Biology). View Reference
      6. Yamakawa N, Ohto U, Akashi-Takamura S, et al. Human TLR4 polymorphism D299G/T399I alters TLR4/MD-2 conformation and response to a weak ligand monophosphoryl lipid A. Int Immunol. 2013; 25(1):45-52. (Immunogen: Bioassay, Blocking, Flow cytometry, Immunoprecipitation, Inhibition). View Reference
      7. Zarember KA, Godowski PJ. Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines. J Immunol. 2002; 168(2):554-561. (Biology). View Reference
      View All (7) View Less
      757134 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.