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      R718 Mouse Anti-Mouse SLAMF9
      R718 Mouse Anti-Mouse SLAMF9
      Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells.  C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062), FITC Rat Anti-Mouse CD19 (Cat. No. 553785), BUV395 Rat Anti-Mouse CD45R/B220 (Cat. No. 563793), BD Horizon™ BV421 Rat Anti-Mouse CD317 (BST2) [Cat. No. 566431] antibodies, and with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 567470; Left Plot) or BD Horizon™ R718 Mouse Anti-Mouse SLAMF9 antibody (Cat. No. 569819/569894; Right Plot) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 (or Ig Isotype control staining) versus CD317 (BST2) was derived by progressive gating on B220/CD45R+ Lineage-negative cells (CD3e-CD19-) with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      R718 Mouse Anti-Mouse SLAMF9
      Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells.  C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062), FITC Rat Anti-Mouse CD19 (Cat. No. 553785), BUV395 Rat Anti-Mouse CD45R/B220 (Cat. No. 563793), BD Horizon™ BV421 Rat Anti-Mouse CD317 (BST2) [Cat. No. 566431] antibodies, and with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 567470; Left Plot) or BD Horizon™ R718 Mouse Anti-Mouse SLAMF9 antibody (Cat. No. 569819/569894; Right Plot) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 (or Ig Isotype control staining) versus CD317 (BST2) was derived by progressive gating on B220/CD45R+ Lineage-negative cells (CD3e-CD19-) with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      CD2F-10; Cd2f10; SLAM family member 9; Slamf9
      Mouse (QC Testing)
      Mouse C57BL/6 IgG1, κ
      Mouse SLAMF9 Fusion Protein
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      98365
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      8. For U.S. patents that may apply, see bd.com/patents.
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      569894 Rev. 1
      Antibody Details
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      M349

      The M349 monoclonal antibody specifically recognizes mouse Signaling lymphocyte activation molecule family member 9 that is also known as SLAM family member 9 (SLAMF9) and CD2 antigen family member 10 (Cd2f10, CD2F-10). SLAMF9 is a type I transmembrane glycoprotein that is encoded by Slamf9 which belongs to the SLAM (CD150)/CD2 subset of the Ig superfamily. SLAMF9 is comprised of an N-terminal extracellular domain that contains an IgV-type domain followed by a IgC2-type domain and a transmembrane region. SLAMF9 has a short lysine- and arginine- rich cytoplasmic domain that lacks Immunoreceptor Tyrosine-based Switch Motifs (ITSMs) characteristic of other SLAM/CD2 subset members. SLAMF9 is variably expressed on plasmacytoid dendritic cells, peritoneal B1 cells and F4/80-low macrophages. SLAMF9 may play a role in the induction of pro-inflammatory cytokines and bacterial clearance.

      569894 Rev. 1
      Format Details
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      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      569894 Rev.1
      Citations & References
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      View product citations for antibody "569894" on CiteAb

      Development References (5)

      1. Cannons JL, Tangye SG, Schwartzberg PL. SLAM family receptors and SAP adaptors in immunity.. Annu Rev Immunol. 2011; 29:665-705. (Biology). View Reference
      2. Dollt C, Michel J, Kloss L, et al. The novel immunoglobulin super family receptor SLAMF9 identified in TAM of murine and human melanoma influences pro-inflammatory cytokine secretion and migration.. Cell Death Dis. 2018; 9(10):939. (Biology). View Reference
      3. Mikulin JA, Bates BL, Wilson TJ. A simplified method for separating renal MPCs using SLAMF9.. Cytometry A. 2021; 99(12):1209-1217. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
      4. Sever L, Radomir L, Stirm K, et al. SLAMF9 regulates pDC homeostasis and function in health and disease.. Proc Natl Acad Sci U S A. 2019; 116(33):16489-16496. (Biology). View Reference
      5. Wilson TJ, Clare S, Mikulin J, et al. Signalling lymphocyte activation molecule family member 9 is found on select subsets of antigen-presenting cells and promotes resistance to Salmonella infection.. Immunology. 2020; 159(4):393-403. (Immunogen: Flow cytometry). View Reference
      View All (5) View Less
      569894 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.