Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Ireland (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      R718 Mouse Anti-Human Siglec-9 (CD329)
      R718 Mouse Anti-Human Siglec-9 (CD329)
      Multiparameter flow cytometric analysis of Siglec-9 (CD329) expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and then preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220). The leucocytes were then stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-Human Siglec-9 (CD329) antibody (Cat. No. 569484/569485; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of Siglec-9 (CD329) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
      R718 Mouse Anti-Human Siglec-9 (CD329)
      Multiparameter flow cytometric analysis of Siglec-9 (CD329) expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and then preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220). The leucocytes were then stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-Human Siglec-9 (CD329) antibody (Cat. No. 569484/569485; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of Siglec-9 (CD329) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
      Product Details
      Down Arrow Up Arrow


      BD Horizon™
      SIGLEC9; siglec-9; CD329
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human Siglec-9-Fc
      Flow cytometry (Routinely Tested)
      5 µl/test
      27180
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      6. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      9. For U.S. patents that may apply, see bd.com/patents.
      Show More blue down arrow Show Less blue up arrow
      569484 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      K8

      The K8 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 9 (Siglec-9) which is also known as CD329. Siglec-9 (CD329) is a single-pass type I transmembrane glycoprotein that is encoded by SIGLEC9 which belongs to the I-type lectins within the Ig superfamily. This lectin is comprised of an N-terminal extracellular region that contains an IgV domain, which binds sialic acid, followed by two Ig-like C2-type domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif . It is highly expressed on monocytes, neutrophils, and expressed at lower levels on a proportion of NK cells, B cells and T cells. Siglec-9 (CD329) may play roles in the regulation of T cell and NK cell responses and neutrophil apoptosis.

      569484 Rev. 1
      Format Details
      Down Arrow Up Arrow
      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      569484 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "569484" on CiteAb

      Development References (5)

      1. Avril T, Floyd H, Lopez F, Vivier E, Crocker PR. The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells.. J Immunol. 2004; 173(11):6841-9. (Clone-specific: Flow cytometry, Functional assay). View Reference
      2. Carlin AF, Uchiyama S, Chang YC, Lewis AL, Nizet V, Varki A. Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response. Blood. 2009; 113(14):3333-3336. (Biology). View Reference
      3. Fraschilla I, Pillai S. Viewing Siglecs through the lens of tumor immunology.. Immunol Rev. 2017; 276(1):178-191. (Biology). View Reference
      4. Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A. Loss of Siglec expression on T lymphocytes during human evolution.. Proc Natl Acad Sci U S A. 2006; 103(20):7765-70. (Biology). View Reference
      5. Zhang JQ, Nicoll G, Jones C, Crocker PR. Siglec-9, a novel sialic acid binding member of the immunoglobulin superfamily expressed broadly on human blood leukocytes.. J Biol Chem. 2000; 275(29):22121-6. (Immunogen: Flow cytometry). View Reference
      View All (5) View Less
      569484 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.