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Purified Rat Anti-Mouse Syndecan-4
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
BCB10 mouse pre-B cell line
Flow cytometry (Routinely Tested), ELISA (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

We recommend the use of Mouse Fc Block™ (anti-mouse CD16/CD32, mAb 2.4G2, Cat. No. 553141/553142) to reduce non-specific staining. For staining in the presence of Mouse Fc Block™, the second-step antibody must not recognize the 2.4G2 antibody (rat IgG2b, κ). Therefore, we recommend biotinylated mouse anti-rat IgG2a (Cat. no. 553894) as a secondary antibody, followed by Streptavidin-PE (Cat. No. 554061).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550350 Rev. 4
Antibody Details
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The KY/8.2 mAb reacts with mouse syndecan-4, a cell-surface heparan sulfate proteoglycan. Although the ligand(s) for this molecule is unknown, syndecans are known to interact with the heparin-binding domain of growth factors, chemokines, and extracellular matrix components. Accordingly, heparin inhibits the binding of syndecan-4 to the pre-B cell line BC7.12. Syndecan-4 is localized to focal adhesions and binds protein-kinase Cα. Thus, it is involved in cytoskeletal organization and signaling. Syndecan-4 is expressed on stromal cells and B-lymphocyte precursors in bone marrow, in addition to most splenic B cells and a small population of T lymphocytes. It is highly expressed on activated B lymphocytes, but is downregulated in isotype-switched memory B cells. The ectodomain of syndecan-4 is shed from cell surfaces constitutively and in response to specific physiological signals, and the resulting soluble fragments are functional. Immobilized KY/8.2 mAb induced the formation of dendritic  processes on syndecan-4-transfected cells and on anti-CD40-activated splenic B cells. Other methods of activating B cells have not produced the same effect. This antibody recognizes an epitope in the N-terminus of syndecan-4, regardless of post-translational modifications.

550350 Rev. 4
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
550350 Rev.4
Citations & References
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Development References (4)

  1. Fitzgerald ML, Wang Z, Park PW, Murphy G, Bernfield M. Shedding of syndecan-1 and -4 ectodomains is regulated by multiple signaling pathways and mediated by a TIMP-3-sensitive metalloproteinase. J Cell Biol. 2000; 148(4):811-824. (Biology). View Reference
  2. Rapraeger AC, Ott VL. Molecular interactions of the syndecan core proteins. Curr Opin Cell Biol. 1998; 10(5):620-628. (Biology). View Reference
  3. Woods A, Couchman JR. Syndecan-4 and focal adhesion function. Curr Opin Cell Biol. 2001; 13(5):578-583. (Biology). View Reference
  4. Yamashita Y, Oritani K, Miyoshi EK, Wall R, Bernfield M, Kincade PW. Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. J Immunol. 1999; 162(10):5940-5948. (Immunogen: ELISA). View Reference
View All (4) View Less
550350 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.