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Purified Rabbit Anti-Human CD88
Purified Rabbit Anti-Human CD88

Immunohistochemistry analysis of CD88 expression on human tonsil paraffin sections. Dendritic cells (Left Panel) or granulocytes (Right Panel) from human tonsil sections were stained with Purified Rabbit Anti-Human CD88 (2.5 µg/ml; Cat. No. 550733), followed by Biotinylated Donkey Anti-Rabbit Ig (5 µg/ml; Jackson ImmunoResearch Cat. No. 711-065-152) and Streptavidin HRP (Cat. No. 550946).

Purified Rabbit Anti-Human CD88

Flow cytometric analysis of CD88 expression on human granulocytes. Whole blood was stained with either Purified Rabbit Anti-Human CD88 (solid line histogram) or Purified Rabbit IgG (Jackson ImmunoResearch Cat. No. 011-000-003; dashed line histogram) at 1 µg/test, followed by FITC Goat Anti-Rabbit IgG (Cat. No. 554020). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 349202). Fluorescent histograms depicting CD88 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

Immunohistochemistry analysis of CD88 expression on human tonsil paraffin sections. Dendritic cells (Left Panel) or granulocytes (Right Panel) from human tonsil sections were stained with Purified Rabbit Anti-Human CD88 (2.5 µg/ml; Cat. No. 550733), followed by Biotinylated Donkey Anti-Rabbit Ig (5 µg/ml; Jackson ImmunoResearch Cat. No. 711-065-152) and Streptavidin HRP (Cat. No. 550946).

Flow cytometric analysis of CD88 expression on human granulocytes. Whole blood was stained with either Purified Rabbit Anti-Human CD88 (solid line histogram) or Purified Rabbit IgG (Jackson ImmunoResearch Cat. No. 011-000-003; dashed line histogram) at 1 µg/test, followed by FITC Goat Anti-Rabbit IgG (Cat. No. 554020). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 349202). Fluorescent histograms depicting CD88 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

Product Details
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BD Pharmingen™
C5a Receptor
Human (QC Testing)
Rabbit IgG
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
AB_393854
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: The purified C85-2506 clone can be used for immunohistochemistry to detect expression of the human C5aR. The antibody has been tested on frozen and fixation of paraffin sections of human tonsil and paraffin sections of human spleen, liver, colon and lung. The dilution range recommended for this antibody is 1-10 to 1-20. The C85-2506 clone does not cross react with C5aR expressed on mouse, rat, pig or monkey tissue. Using biotinylated donkey anti-rabbit Ig (Jackson ImmunoResearch) as the secondary antibody, followed by a peroxidase-based detection system, the expression of C5aR was observed on dendritic cells, granulocytes and monocytes of human tonsil.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550733 Rev. 2
Antibody Details
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C85-2506

The human C5a receptor (C5aR) is expressed on granulocytes, monocytes, macrophages mast cells human cell lines of myeloid origin, and certain cell types in several organs, including dendritic cells of secondary lymphoid organs, liver  hepatocytes, lung bronchial and alveolar epithelial cells, lung vascular smooth muscle, endothelial cells astrocytes, microglia, and fibroblast-like cells of the brain. The C85-2506 is a rabbit monoclonal antibody generated by fusion of splenocytes from rabbits immunized with human C5aR peptides and the rabbit fusion partner 240E-1.

550733 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550733 Rev.2
Citations & References
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Development References (10)

  1. Fayyazi A, Sandau R, Duong LQ. C5a receptor and interleukin-6 are expressed in tissue macrophages and stimulated keratinocytes but not in pulmonary and intestinal epithelial cells. Am J Pathol. 1999; 154(2):495-501. (Biology). View Reference
  2. Füreder W, Agis H, Willheim M. Differential expression of complement receptors on human basophils and mast cells. Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells. J Immunol. 1995; 155(6):3152-3160. (Biology). View Reference
  3. Gasque P, Singhrao SK, Neal JW, Götze O, Morgan BP. Expression of the receptor for complement C5a (CD88) is up-regulated on reactive astrocytes, microglia, and endothelial cells in the inflamed human central nervous system. Am J Pathol. 1997; 150(1):31-41. (Biology). View Reference
  4. Haviland DL, McCoy RL, Whitehead WT, et al. Cellular expression of the C5a anaphylatoxin receptor (C5aR): Demonstration of C5aR on nonmyeloid cells of the liver and lung. J Immunol. 1995; 154:1861-1869. (Biology).
  5. Morelli A, Larregina A, Chuluyán I, Kolkowski E, Fainboim L. Expression and modulation of C5a receptor (CD88) on skin dendritic cells. Chemotactic effect of C5a on skin migratory dendritic cells. Immunology. 1996; 89(1):126-134. (Biology). View Reference
  6. Nataf S, Davoust N, Barnum SR. Kinetics of anaphylatoxin C5a receptor expression during experimental allergic encephalomyelitis. J Neuroimmunol. 1998; 91(1-2):147-155. (Biology). View Reference
  7. Paradisis PM, Campbell IL, Barnum SR. Elevated complement C5a receptor expression on neurons and glia in astrocyte-targeted interleukin-3 transgenic mice. Glia. 1998; 24(3):338-345. (Biology). View Reference
  8. Spieker-Polet H, Sethupathi P, Yam PC, Knight KL. Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas. Proc Natl Acad Sci U S A. 1995; 92(20):9348-9352. (Immunogen). View Reference
  9. Stahel PF, Frei K, Eugster HP. TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis. J Immunol. 1997; 159(2):861-869. (Biology). View Reference
  10. Wetsel RA. Expression of the complement C5a anaphylatoxin receptor (C5aR) on non-myeloid cells. Immunol Lett. 1995; 44(2-3):183-187. (Biology). View Reference
View All (10) View Less
550733 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.