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Purified Mouse Anti-Rat High Affinity IgE Receptor
Purified Mouse Anti-Rat High Affinity IgE Receptor

Expression of FcεRI on RBL cells. RBL cells were incubated with either Purified Mouse IgG1, κ isotype control (Cat. no. 557273, open dash line overlay) or purified BC4 mAb (shaded histogram), followed by biotinylated F(ab')2 rat anti-mouse IgG and finally Streptavidin-FITC (Cat. no. 554060). The cells in both the shaded histogram and the overlay were gated on 7-AAD negative cells and then the expression of FcεRI is depicted. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Expression of FcεRI on RBL cells. RBL cells were incubated with either Purified Mouse IgG1, κ isotype control (Cat. no. 557273, open dash line overlay) or purified BC4 mAb (shaded histogram), followed by biotinylated F(ab')2 rat anti-mouse IgG and finally Streptavidin-FITC (Cat. no. 554060). The cells in both the shaded histogram and the overlay were gated on 7-AAD negative cells and then the expression of FcεRI is depicted. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Product Details
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BD Pharmingen™
FcεRI
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Rat basophilic leukemia cell line
Flow cytometry (Routinely Tested), Immunoprecipitation, Inhibition (Reported)
0.5 mg/ml
AB_394210
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Other reported applications include immunoprecipitation, inhibition of IgE binding, and degranulation of rat mast cells by antibody crosslinking.  It is recommended that for immunoflourescent staining of rat cells, the BC4 antibody be carefully titrated and used with F(ab')2  secondary reagents to reduce cellular degranulation.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. An isotype control should be used at the same concentration as the antibody of interest.
551469 Rev. 4
Antibody Details
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BC4

The BC4 antibody reacts with the high-affinity IgE Fc receptor (FcεRI) expressed in rat mast cells, basophils, and non-B non-T cells.  The rat FcεRI is expressed as a tetrameric molecule consisting of one α chain, one β chain, and two identical γ chains. The BC4 antibody has been reported to detect all three chains of the FcεRI by immunoprecipitation.  In the rat model, the β chain (FcRβ) is required for cell-surface expression of the complete FcεRI. This differs from the human FcεRI, which can be expressed in trimer (αγ2) or tetramer forms (βγ2).  Furthermore, the FcRβ has been characterized as a potent signaling molecule capable of substantially amplifying Fc-mediated cellular responses. The FcεRI functions to mediate cellular degranulation and the release of histamine, leukotrienes, and various cytokines and chemokines. The structure, expression, function, and signaling mechanisms of the FcεRI have been reviewed.

551469 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
551469 Rev.4
Citations & References
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Development References (3)

  1. Basciano LK, Berenstein EH, Kmak L, Siraganian RP. Monoclonal antibodies that inhibit IgE binding. J Biol Chem. 1986; 261(25):11823-11831. (Immunogen: Immunoprecipitation, Inhibition). View Reference
  2. Dombrowicz D, Nutten S, Desreumaux P. Role of the high affinity immunoglobulin E receptor in bacterial translocation and intestinal inflammation. J Exp Med. 2001; 193(1):25-34. (Biology). View Reference
  3. Kinet JP. The high-affinity IgE receptor (Fc epsilon RI): from physiology to pathology. Annu Rev Immunol. 1999; 931-972. (Clone-specific: Immunofluorescence). View Reference
551469 Rev. 4

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.