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      Purified Mouse Anti-Rat CD24
      Purified Mouse Anti-Rat CD24
      Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Purified Mouse Anti-Rat CD24
      Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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      BD Pharmingen™
      HSA; Heat-stable antigen; Nectadrin; Cd24a; Signal transducer CD24
      Rat (QC Testing)
      Mouse BALB/c IgM, κ
      Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen, Western blot (Reported)
      0.5 mg/ml
      AB_394064
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      551133 Rev. 5
      Antibody Details
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      HIS50

      The HIS50 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers and thymus. The HIS50 antibody detects CD24 on most B-lineage cells in the bone marrow, on most highly surface IgM (sIgM)-positive B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on B cells that expresses low levels of sIgM and on follicular B lymphocytes. The HIS50 antibody does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody crossreacts with intracellular antigen(s) in human lymphocytes.

      551133 Rev. 5
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      551133 Rev.5
      Citations & References
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      Development References (3)

      1. Akashi T, Shirasawa T, Hirokawa K.. Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines.. Virchows Arch. 1994; 425(4):399-406. (Biology: Bioassay). View Reference
      2. Hermans MH, Deenen GJ, De Boer N, Bo W, Kroese FG, Opstelten D. Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat. Immunology. 1997; 90(1):14-22. (Immunogen: Immunofluorescence, Immunohistochemistry). View Reference
      3. Pearce EI, Ammerlaan WA, Opstelten D.. Characterization of rat HSA/CD24 protein bearing the B lineage-restricted epitope recognized by MAb HIS50.. Hybridoma. 1996 February; 15(1):33-42. (Clone-specific: Western blot). View Reference
      551133 Rev. 5

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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