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Purified Mouse Anti-Rat CD24
Purified Mouse Anti-Rat CD24

Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Product Details
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BD Pharmingen™
HSA; Heat-stable antigen; Nectadrin; Cd24a; Signal transducer CD24
Rat (QC Testing)
Mouse BALB/c IgM, κ
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen, Western blot (Reported)
0.5 mg/ml
AB_394064
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551133 Rev. 5
Antibody Details
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HIS50

The HIS50 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers and thymus. The HIS50 antibody detects CD24 on most B-lineage cells in the bone marrow, on most highly surface IgM (sIgM)-positive B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on B cells that expresses low levels of sIgM and on follicular B lymphocytes. The HIS50 antibody does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody crossreacts with intracellular antigen(s) in human lymphocytes.

551133 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
551133 Rev.5
Citations & References
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Development References (3)

  1. Akashi T, Shirasawa T, Hirokawa K.. Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines.. Virchows Arch. 1994; 425(4):399-406. (Biology: Bioassay). View Reference
  2. Hermans MH, Deenen GJ, De Boer N, Bo W, Kroese FG, Opstelten D. Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat. Immunology. 1997; 90(1):14-22. (Immunogen: Immunofluorescence, Immunohistochemistry). View Reference
  3. Pearce EI, Ammerlaan WA, Opstelten D.. Characterization of rat HSA/CD24 protein bearing the B lineage-restricted epitope recognized by MAb HIS50.. Hybridoma. 1996 February; 15(1):33-42. (Clone-specific: Western blot). View Reference
551133 Rev. 5

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.