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Purified Mouse Anti-Rat CD147
Product Details
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BD Pharmingen™
CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin; OX-47 Antigen
Rat (QC Testing)
Mouse IgG1, κ
Rat T blasts
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
0.5 mg/ml
AB_2066968
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
552012 Rev. 3
Antibody Details
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OX-47

The OX-47 monoclonal antibody specifically recognizes rat CD147 (previously known as OX-47  antigen), a 54-kDa (unreduced) or 65-kDa (reduced) Ig superfamily protein with an unusual single-transmembrane domain. CD147 is the rat homolog of the human EMMPRIN, mouse gp42 or basigin, and the avian neurothelin or HT7 molecules. The CD147 molecule is widely expressed on both hematopoietic and non-hematopoietic cells and tissues. It is expressed on bone-marrow cells, thymocytes, some resting lymphocytes, peritoneal macrophages, and various epithelial cells and is strongly upregulated in activated T and B cells. CD147 has been detected in heart muscle cells, muscle tissues, and endothelium of brain capillaries and kidney tubules. The function of rat CD147 has not been characterized, but it is thought to play a role in cellular adhesion.

552012 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
552012 Rev.3
Citations & References
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Fossum S, Mallett S, Barclay AN. The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence. Eur J Immunol. 1991; 21(3):671-679. (Biology). View Reference
  3. Paterson DJ, Jefferies WA, Green JR. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987; 24(12):1281-1290. (Immunogen). View Reference
552012 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.