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Purified Mouse Anti-Human Granzyme K
Purified Mouse Anti-Human Granzyme K
Flow cytometric analysis of Granzyme K expression on human peripheral blood mononuclear cells (PBMCs). PBMCs were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 554126; dashed line histogram) or Purified Mouse Anti-Human Granzyme K (Cat. No. 567938; solid line histogram) at 1.0 µg/test, followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). The fluorescent histogram showing Granzyme K expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Granzyme K expression on human peripheral blood mononuclear cells (PBMCs). PBMCs were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 554126; dashed line histogram) or Purified Mouse Anti-Human Granzyme K (Cat. No. 567938; solid line histogram) at 1.0 µg/test, followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). The fluorescent histogram showing Granzyme K expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
GZMK; GRAK; TRYP2; fragmentin-3; granzyme 3; tryptase II
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human Granzyme K Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
567938 Rev. 1
Antibody Details
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G3H69

The G3H69 monoclonal antibody specifically recognizes Granzyme K which is also known as GRAK, Granzyme-3, Fragmentin-3, Tryptase II (TRYP2), or NK-tryptase-2 (NK-Tryp-2). This granule-associated enzyme is a serine protease that cleaves peptides after the basic residues lysine and arginine. Granzyme K is encoded by GZMK (granzyme K) that belongs to the Granzyme subfamily (Granzymes A, B, H, K, M) within the peptidase S1 family. Granzyme K is expressed in the granules of CD56-bright natural killer (NK) cells, NKT cells, γδ T cells, and cytotoxic CD4+ T cells and CD8+ T cells that can kill pathogen-infected or tumor cells. Granzyme K can activate the SET complex enabling DNase NM23-H1 to make single-stranded nicks in chromosomal DNA which ultimately contribute to target cell death. Granzyme K can also act on Bid and p53 to activate certain cell death pathways. Elevated levels of Granzyme K have been observed in the blood and bodily fluids from individuals under certain infectious or inflammatory conditions.

        

567938 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
567938 Rev.1
Citations & References
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View product citations for antibody "567938" on CiteAb

Development References (5)

  1. Bade B, Boettcher HE, Lohrmann J, et al. Differential expression of the granzymes A, K and M and perforin in human peripheral blood lymphocytes.. Int Immunol. 2005; 17(11):1419-28. (Biology). View Reference
  2. Hameed A, Lowrey DM, Lichtenheld M, Podack ER. Characterization of three serine esterases isolated from human IL-2 activated killer cells. J Immunol. 1988; 141:3142-3147. (Biology). View Reference
  3. Inagaki H, Hirata Y, Shimizu T, Kobayashi M, Li Q, Kawada T. Expression of granzyme 3 protein in human peripheral blood lymphocytes analyzed by flow cytometry (PP-108-30). Int Immunol (Abstract). 2010; 22:172. (Immunogen: Flow cytometry).
  4. Lieberman J. The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal.. Nat Rev Immunol. 2003; 3(5):361-70. (Biology). View Reference
  5. Wilharm E, Parry MA, Friebel R, et al. Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma. J Biol Chem. 1999; 274:27331-27337. (Biology). View Reference
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567938 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.