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Purified Hamster Anti-Mouse IL-1α
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Armenian Hamster IgG, λ
Mouse IL-1α recombinant protein
ELISA Capture (Routinely Tested), Intracellular staining (flow cytometry), Neutralization (Tested During Development), Western blot (Reported)
0.5 mg/ml
AB_393776
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified ALF-161 antibody (Cat. No. 550604) is useful as a capture antibody for a sandwich ELISA for measuring mouse IL-1α protein levels. Purified ALF-161 antibody can be paired with the biotinylated polyclonal IgG fraction of rabbit anti-mouse IL-1α (Cat. No. 550606) as the detecting antibody, with recombinant mouse IL-1α as the standard. Purified ALF-161 antibody should be titrated 1-4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse IL-1α ranging from ~1000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

Note: This ELISA antibody pair shows no crossreactivity with other purified recombinant cytokines or receptors that were tested including:  Mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-16, IL-17, GM-CSF, IFN-γ, TNF, TNFRI, TNFRII, MCP-1, CRG

Human IL-1α, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-16, GM-CSF, G-CSF, TNF, LT-α, TNFRI, TNFRII, MCP-1, MCP-2, IFN-γ, RANTES, MIG

Rat IL-1α, IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, MCP-1, TNF.

Western Blot: The purified ALF-161 antibody has been found useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.

Blocking Control for Intracellular Staining: The purified ALF-161 antibody (Cat. No. 550604) can be used as a blocking control to demonstrate specificity of IL-1α staining by PE-labeled ALF-161 antibody (Cat. No. 559810). To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 5-10 µg of unlabeled ALF-161 antibody (Cat. No. 550604) for 15 minutes  at 4°C, prior to staining with PE-labeled ALF-161 antibody (eg ≤ 0.12 µg mAb/1 million cells). The intracellular staining technique and the use of blocking controls have been described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site,www.bdbiosciences.com.

Product Notices

  1. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550604 Rev. 1
Antibody Details
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ALF-161

This antibody recognizes the precursor, secreted and membrane-associated forms of mouse interleukin-1α (IL-1α) protein. No cross-reactivity was detected with mouse IL-1β. This antibody does not recognize human IL-1α or IL-1β. The cross-reactivity of this antibody with IL-1α from other species has not been tested. The immunogen used to generate this ALF-161 hybridoma was purified, recombinant mouse IL-1α protein. This is a neutralizing antibody.

550604 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550604 Rev.1
Citations & References
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Development References (5)

  1. Chang MJ, Modzelewski RA, Russell DM, Johnson CS. Interleukin 1 alpha and gamma-interferon induction of nitric oxide production from murine tumor-derived endothelial cells. Cancer Res. 1996; 56(4):886-891. (Biology). View Reference
  2. Fuhlbrigge RC, Sheehan KC, Schreiber RD, Chaplin DD, Unanue ER. Monoclonal antibodies to murine IL-1 alpha. Production, characterization, and inhibition of membrane-associated IL-1 activity. J Immunol. 1988; 141(8):2643-2650. (Clone-specific: Neutralization). View Reference
  3. Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Clone-specific: Neutralization). View Reference
  4. Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Clone-specific: Neutralization). View Reference
  5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). View Reference
View All (5) View Less
550604 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.