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Purified Hamster Anti-Mouse gp49 Receptor
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Armenian Hamster IgG3, κ
gp49B-Fc fusion protein composed of extracelluar domain of gp49B
Flow cytometry (Routinely Tested), ELISA, Immunoprecipitation, Induction (Reported)
0.5 mg/ml
AB_394384
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

For optimal staining, we recommend the use of biotinylated anti-hamster IgG mAb G192-1 (Cat. no. 554025) as the second-step antibody, followed by a bright third-step reagent such as Streptavidin-APC (Cat. no. 554067) or Streptavidin-PE (Cat. no. 554061).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
552420 Rev. 3
Antibody Details
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H1.1

The H1.1 monoclonal antibody specifically reacts with gp49B, a structural homolog of the human Killer Inhibitory Receptors (KIR), and with the truncated related molecule gp49A.  The gp49 receptors are expressed on myeloid cells of adult bone marrow, spleen, and liver and fetal liver.  They are not found on resting lymphoid cells, but their expression is upregulated on activated NK cells.  gp49B is a member of the Ig superfamily containing two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) domains, while gp49A has no ITIM.  Although its ligand is unknown, it is established that cross-linking of gp49B can suppress cell activation events.

552420 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
552420 Rev.3
Citations & References
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Development References (5)

  1. Katz HR, Vivier E, Castells MC, McCormick MJ, Chambers JM, Austen KF. Mouse mast cell gp49B1 contains two immunoreceptor tyrosine-based inhibition motifs and suppresses mast cell activation when coligated with the high-affinity Fc receptor for IgE. Proc Natl Acad Sci U S A. 1996; 93(20):10809-10814. (Biology). View Reference
  2. McCormick MJ, Castells MC, Austen KF, Katz HR. The gp49A gene has extensive sequence conservation with the gp49B gene and provides gp49A protein, a unique member of a large family of activating and inhibitory receptors of the immunoglobulin superfamily. Immunogenetics. 1999; 50(5-6):286-294. (Biology). View Reference
  3. Wang LL, Blasioli J, Plas DR, Thomas ML, Yokoyama WM. Specificity of the SH2 domains of SHP-1 in the interaction with the immunoreceptor tyrosine-based inhibitory motif-bearing receptor gp49B. J Immunol. 1999; 162(3):1318-1323. (Immunogen). View Reference
  4. Wang LL, Chu DT, Dokun AO, Yokoyama WM. Inducible expression of the gp49B inhibitory receptor on NK cells. J Immunol. 2000; 164(10):5215-5220. (Immunogen: Induction). View Reference
  5. Wang LL, Mehta IK, LeBlanc PA, Yokoyama WM. Mouse natural killer cells express gp49B1, a structural homologue of human killer inhibitory receptors. J Immunol. 1997; 158(1):13-17. (Biology). View Reference
View All (5) View Less
552420 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.