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PE Mouse anti-mouse Ly-49F
PE Mouse anti-mouse Ly-49F

Two-color analysis of Ly-49F expression on splenic NK cells. C57BL/6 splenocytes were simultaneously stained with APC-conjugated mAb PK136 (anti-mouse NK-1.1, Cat. no. 557391, both panels) and PE-conjugated mAb MOPC-31C (mouse IgG1, κ isotype control, Cat. no. 550617, Left Panel) or PE-conjugated mAb HBF-719 (Right Panel).

Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).

Two-color analysis of Ly-49F expression on splenic NK cells. C57BL/6 splenocytes were simultaneously stained with APC-conjugated mAb PK136 (anti-mouse NK-1.1, Cat. no. 557391, both panels) and PE-conjugated mAb MOPC-31C (mouse IgG1, κ isotype control, Cat. no. 550617, Left Panel) or PE-conjugated mAb HBF-719 (Right Panel).

Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Mouse (QC Testing)
Mouse IgG1, κ
CHO-K1 cells transfected with the B6 allele of the Ly-49F gene,1 Klra62 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
16637
AB_393998
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550987 Rev. 3
Antibody Details
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HBF-719

The HBF-719 antibody reacts with the B6 alloantigen of Ly-49F, an inhibitory receptor which is expressed on subsets of natural killer (NK) cells, NK-1.1+ (or DX5+) T lymphocytes (NK-T cells), and on a population of memory CD8+ T lymphocytes in C57BL/6 mice, 1 but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice.3 The Ly-49 family of NK-cell receptors are disulfide linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains (CRD). The Ly-49 family members are expressed independently, such that an individual NKor T-cell may display more than one class of Ly-49 receptor homodimers. Ly-49F has an ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) in its cytoplasmic tail. Therefore, it is probably an inhibitory receptor. It weakly binds to cells expressing the H-2d MHC class I alloantigens.

550987 Rev. 3
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
550987 Rev.3
Citations & References
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Development References (5)

  1. Coles MC, McMahon CW, Takizawa H, Raulet DH. Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. Eur J Immunol. 2000; 30(1):236-244. (Immunogen). View Reference
  2. Hanke T, Takizawa H, McMahon CW, et al. Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors. Immunity. 1999; 11(1):67-77. (Biology). View Reference
  3. Raulet DH, Held W, Correa I, Dorfman JR, Wu MF, Corral L. Specificity, tolerance and developmental regulation of natural killer cells defined by expression of class I-specific Ly49 receptors. Immunol Rev. 1997; 155:41-52. (Biology). View Reference
  4. Takei F, Brennan J, Mager DL. The Ly-49 family: genes, proteins and recognition of class I MHC. Immunol Rev. 1997; 155:67-77. (Biology). View Reference
  5. The Jackson Laboratory. Mouse Genome Database (MGD), Mouse Genome Informatics Web Site. Available: http://www.informatics.jax.org September, 2003.
View All (5) View Less
550987 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.