.png){kind=spacer}
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
The NFLD.D1 monoclonal antibody is specific for the HLA-DR4 serogroup. This panspecific HLA-DR4 antibody binds to an epitope expressed in the extracellular beta-2 (β2) domain of all HLA-DRB1 beta chain variants encoded by HLA-DRB1*04 alleles. The epitope recognized by the NFLD.D1 antibody depends on both a leucine present at sequence position 180 and a threonine at position 181. HLA-DR antigens are heterodimers comprised of type I transmembrane glycoprotein alpha and beta subunits that contain two extracellular domains, transmembrane regions, and cytoplasmic tails. The ~34 kDa HLA-DR alpha (HLA-DRα) chain is encoded by HLA-DRA whereas the alternative ~28 kDa HLA-DR beta (HLA-DRβ) chains are encoded by one of the four different HLA-DRB loci (HLA-DRB1,3,4, and 5) that are located within the Human Leukocyte Antigen (HLA) Complex of chromosome 6. HLA-DR is variably expressed on antigen-presenting cells (APCs), such as dendritic cells, B lymphocytes, monocytes, macrophages, and thymic epithelial cells as well as by activated T cells, epithelial cells, inflammatory fibroblasts, and tumor cells. Polymorphisms in the HLA-DRβ chains allow for variability in peptide binding and the presentation of self and foreign peptide antigens to CD4+ T lymphocytes that generate and regulate adaptive immune responses. The NFLD.D1 antibody can be especially useful along with other HLA-DRβ allotype-specific antibodies in applications such as immunofluorescence and flow cytometry for analyzing allelic HLA-DRB expression patterns by individual cells within heterogeneous cell populations. Some HLA-DRB1*04 alleles or their aberrant expression patterns have been associated with susceptibility to diseases including rheumatoid arthritis.
Development References (7)
-
Drover S, Karr RW, Fu XT, Marshall WH. Analysis of monoclonal antibodies specific for unique and shared determinants on HLA-DR4 molecules.. Hum Immunol. 1994; 40(1):51-60. (Immunogen: ELISA, Flow cytometry). View Reference
-
Drover S, Kovats S, Masewicz S, Blum JS, Nepom GT. Modulation of peptide-dependent allospecific epitopes on HLA-DR4 molecules by HLA-DM.. Hum Immunol. 1998; 59(2):77-86. (Clone-specific: Flow cytometry). View Reference
-
Fu XT, Drover S, Marshall WH, Karr RW. HLA-DR residues accessible under the peptide-binding groove contribute to polymorphic antibody epitopes.. Hum Immunol. 1995; 43(4):243-50. (Clone-specific: Flow cytometry). View Reference
-
Marshall, W. H., S. Drover, et al. Assessing prognosis in rheumatoid arthritis using monoclonal antibodies and flow cytometry. In: S. Drover. Madrigal A.J., Bencová M., Middleton D., Charron D., Nánási T., ed. Immunogenetics: Advances and Education. Dordrecht: Springer; 1997:87-98.
-
Patil NS, Hall FC, Drover S, et al. Autoantigenic HCgp39 epitopes are presented by the HLA-DM-dependent presentation pathway in human B cells.. J Immunol. 2001; 166(1):33-41. (Clone-specific: Flow cytometry). View Reference
-
Patil NS, Pashine A, Belmares MP, et al. Rheumatoid arthritis (RA)-associated HLA-DR alleles form less stable complexes with class II-associated invariant chain peptide than non-RA-associated HLA-DR alleles.. J Immunol. 2001; 167(12):7157-68. (Clone-specific: Flow cytometry). View Reference
-
Spurrell DR, Oldford SA, Frost T, et al. Discordant expression of HLA class II-associated co-chaperones and HLA-DRB alleles in cultured fibroblast-like synoviocytes.. Hum Immunol. 2004; 65(12):1516-29. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.