PE Mouse Anti-Human Chondroitin Sulfate (NG2)

BD Pharmingen™ PE Mouse Anti-Human Chondroitin Sulfate (NG2)

Name: PE Mouse Anti-Human Chondroitin Sulfate (NG2)
Brand: PE Mouse Anti-Human Chondroitin Sulfate (NG2)
SKU: 569963

Clone 7.1 (RUO)

Flow cytometric analysis of Chondroitin Sulfate (NG2) expression on Human M21 cells. Cells from the Human M21 (Melanoma) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histograms) or PE Mouse Anti-Chondroitin Sulfate (NG2) antibody (Cat. No. 569962/569963; solid line histogram) at 0.125 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Chondroitin Sulfate (NG2) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD Celesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CSPG4; HMW-MAA; MCSP; MCSPG; NG2

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human BM stromal cell line established by infection with SV-40

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 1464

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.
An isotype control should be used at the same concentration as the antibody of interest.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Human BD Fc Block™ RUO

Size 0.25 mg Cat No. 564220

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

DAPI Solution RUO

Size 1 mg Cat No. 564907

569963 Rev. 1

Antibody Details

7.1

The 7.1 monoclonal antibody specifically recognizes Neural/Glial Antigen 2 (NG2) which is also known as Melanoma chondroitin sulfate proteoglycan (MCSP) and High molecular weight-melanoma associated antigen (HMW-MAA). This ~251 kDa transmembrane proteoglycan is encoded by CSPG4 (Chondroitin sulfate proteoglycan 4). Chondroitin Sulfate (NG2) is expressed by glial cells but not detected on normal marrow hematopoietic and peripheral blood cells. It is highly expressed by cells in some cancers including melanomas, leukemias, sarcomas and carcinomas. Chondroitin Sulfate (NG2) plays important roles in cellular proliferation, migration, and in oncogenic pathways involved in malignant transformation and metastases.

569963 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

569963 Rev.1

Citations & References

View product citations for antibody "569963" on CiteAb

Development References (5)

Behm FG, Smith FO, Raimondi SC, Pui CH, Bernstein ID. Human homologue of the rat chondroitin sulfate proteoglycan, NG2, detected by monoclonal antibody 7.1, identifies childhood acute lymphoblastic leukemias with t(4;11)(q21;q23) or t(11;19)(q23;p13) and MLL gene rearrangements.. Blood. 1996; 87(3):1134-9. (Immunogen: Flow cytometry). View Reference
Luo W, Wang X, Kageshita T, Wakasugi S, Karpf AR, Ferrone S. Regulation of high molecular weight-melanoma associated antigen (HMW-MAA) gene expression by promoter DNA methylation in human melanoma cells.. Oncogene. 2006; 25(20):2873-84. (Biology). View Reference
Pluschke G, Vanek M, Evans A, et al. Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan. Proc Natl Acad Sci U S A. 1996; 93(18):9710-9715. (Biology). View Reference
Tamburini E, Dallatomasina A, Quartararo J, et al. Structural deciphering of the NG2/CSPG4 proteoglycan multifunctionality.. FASEB J. 2019; 33(3):3112-3128. (Biology). View Reference
Wang X, Wang Y, Yu L, et al. CSPG4 in cancer: multiple roles.. Curr Mol Med. 2010; 10(4):419-29. (Biology). View Reference

View All (5)

569963 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.